咖啡酸苯乙酯对2型糖尿病大鼠肝脏保护作用的研究

发布时间：2018-02-25 21:13

  本文关键词： 咖啡酸苯乙酯 型糖尿病 氧化应激 肝脏　出处：《时珍国医国药》2016年09期 　论文类型：期刊论文

【摘要】：目的探讨咖啡酸苯乙酯(CAPE)对2型糖尿病(T2DM)大鼠肝脏的保护作用及其作用机制。方法 24只雄性SD大鼠随机分为3组,即空白组,损伤组,保护组。高脂高糖饲料喂养联合链脲佐菌素诱导建立2型糖尿病大鼠模型,建模成功后,保护组大鼠CAPE灌胃一个月,测量血清中总胆固醇(TC)和甘油三酯(TG)的含量,并对肝脏中丙二醛(MDA)、蛋白羰基化(PCO)的含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性进行测定。结果损伤组血清中总胆固醇(TC)和甘油三酯(TG)明显上升,说明糖尿病大鼠血脂升高。在损伤组中MDA、PCO含量明显升高,而保护组MDA、PCO的含量较损伤组减少(P0.05),说明糖尿病能够增加肝脏的氧化应激,诱导氧化损伤,而咖啡酸苯乙酯的保护能够降低MDA、PCO的含量,减少氧化损伤;而SOD、CAT在损伤组中含量降低,可能是机体出现的一种自我保护反应,使得机体本身的抗氧化物质大量消耗,咖啡酸苯乙酯可以减少SOD、CAT含量的增加,保护作用比较明显。结论糖尿病的发生可能增加机体的氧化应激,咖啡酸苯乙酯能够有效降低其氧化损伤程度,从而起到对糖尿病肝脏的保护作用。
[Abstract]:Objective to investigate the protective effect and mechanism of capeic acid (Cape) on the liver of rats with type 2 diabetes mellitus T2DM.Methods Twenty-four male SD rats were randomly divided into three groups: blank group and injury group. The model of type 2 diabetic rats was established by high fat and high sugar diet plus streptozotocin. After the model was successfully established, the rats in the protective group were fed with CAPE for one month, and the contents of serum total cholesterol (TC) and triglyceride (TG) were measured. The contents of malondialdehyde (MDAA), protein carbonylation (PCOs) and the activities of superoxide dismutase (SOD) and catalase (CAT) in liver were determined. Results the serum total cholesterol (TCC) and triglyceride (TG) in the injured group were significantly increased. The results showed that the content of MDA-PCO in the injured group was significantly higher than that in the injured group, while the content of MDA-PCO in the protective group was lower than that in the injured group, indicating that diabetes could increase the oxidative stress of the liver and induce oxidative damage. However, the protection of phenethyl caffeate can reduce the content of MDA-PCO and reduce the oxidative damage, while the decrease of SODP-cat in the injured group may be a self-protective reaction, which causes the body to consume a lot of antioxidants. Conclusion the occurrence of diabetes may increase oxidative stress, and phenethyl caffeic acid can effectively reduce the degree of oxidative damage. Thus plays a protective role in diabetic liver.
【作者单位】： 陕西科技大学生命与工程学院;西安科技大学化学与化工学院;
【基金】：国家自然科学基金(No.21407104;No.81402815)
【分类号】：R587.2
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本文编号：1535161