Toll样受体3对高脂饮食小鼠棕色脂肪组织中解偶联蛋白1表达的影响

发布时间：2018-02-26 09:53

本文关键词： Toll样受体棕色脂肪组织高脂饮食肥胖解偶联蛋白　出处：《现代预防医学》2017年13期 　论文类型：期刊论文

【摘要】：目的探究Toll样受体3(Toll like receptor 3,TLR3)对高脂饮食诱导小鼠棕色脂肪组织中解偶联蛋白1(uncoupling protein 1,UCP-1)表达的影响。方法将雄性C57BL/6小鼠和TLR3基因敲除小鼠各14只分别饲以正常饮食(normal diet,ND)和高脂饮食(high-fat diet,HFD),为期15周。在造模前和15周末对小鼠的体重进行称量。用HE(Hematoxylin and eosin)染色、免疫组化、实时定量PCR方法和Western blot法检测棕色脂肪组织形态和UCP-1表达情况。结果 (1)高脂饮食末,野生型高脂饮食(wildtype-high-fat diet,WT-HFD)组小鼠体重和棕色脂肪组织重量明显高于野生型正常饮食(wildtype-normal diet,WT-ND)组,上述改变被TLR3基因敲除所阻断。(2)与WT-ND组相比,WT-HFD组小鼠棕色脂肪组织中UCP-1的mRNA水平显著升高;与WT-HFD组相比,TLR3-HFD组的UCP-1的mRNA水平有升高趋势;(3)与WT-ND组相比,WT-HFD组小鼠棕色脂肪组织中UCP-1的蛋白水平明显升高;与WT-HFD组相比,TLR3-HFD组的UCP-1的蛋白水平显著升高,过氧化物酶体增殖物激活受体γ辅助激活因子1α(peroxisome proliferator activated receptorγcoactivator-1α,PGC-1α)的蛋白水平有升高趋势。结论TLR3通过抑制棕色脂肪组织的UCP-1的表达,参与高脂饮食引起的肥胖。
[Abstract]:Objective to investigate the effect of Toll like receptor 3 (TLR3), a Toll like receptor, on the expression of uncoupled protein 1 uncoupling protein 1 (UCP-1) in brown adipose tissue of mice induced by high fat diet. Methods male C57BL / 6 mice and TLR3 gene knockout mice were fed with normal diet respectively. The mice were weighed before and at the end of the 15th week with HE(Hematoxylin and eosin. Immunohistochemistry, real-time quantitative PCR and Western blot were used to detect the morphology and UCP-1 expression of brown adipose tissue. The body weight and brown adipose tissue weight of wild type high-fat diettidine WT-HFD-treated mice were significantly higher than those of wild type normal dietate WT-NDs group, the weight of brown adipose tissue in wild type high fat diet group was significantly higher than that in wild type normal diet group (WT-HFDH) group. These changes were blocked by TLR3 knockout.) compared with WT-ND group, the mRNA level of UCP-1 in brown adipose tissue of WT-HFD mice was significantly higher than that of WT-ND group. Compared with WT-HFD group, the mRNA level of UCP-1 in TLR3-HFD group was higher than that of WT-ND group, and the protein level of UCP-1 in brown adipose tissue of TLR3-HFD group was significantly higher than that of WT-ND group, and the UCP-1 protein level of TLR3-HFD group was significantly higher than that of WT-HFD group. The protein level of peroxisome proliferator activated receptor 纬 coactivator-1 伪 (PGC-1 伪) of peroxisome proliferator activated receptor 纬 -coactivator 1 伪 was increased. Conclusion TLR3 may play an important role in obesity induced by high fat diet by inhibiting the expression of UCP-1 in brown adipose tissue.
【作者单位】：浙江中医药大学基础医学院;杭州师范大学医学院;新疆维吾尔自治区人民医院;
【基金】：国家自然科学基金青年基金(81402646) 杭州市科技计划项目(20140633B36)
【分类号】：R589.2

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