我国部分地区静脉吸毒人群HCV基因型分布的探讨

发布时间：2018-02-27 13:02

本文关键词： 丙型肝炎病毒静脉吸毒人群基因型分布　出处：《中国艾滋病性病》2017年08期 　论文类型：期刊论文

【摘要】：目的了解静脉吸毒人群丙型肝炎病毒(HCV)感染者的基因亚型分布及影响因素,为防治静脉吸毒人群感染HCV提供依据。方法收集828份静脉吸毒人群样本,HCV抗体初筛用酶联免疫吸附试验(ELISA),有反应性的样本再用重组免疫印迹法(RIBA)进行补充试验,阳性的样本进行核酸检测;核酸阳性的样本先用HCV基因分型试剂盒进行分型,未通过分型试剂盒确定型别的样本,再用巢式聚合酶链式反应扩增HCV CORE区进行测序,以确定HCV基因型。结果 828份静脉吸毒人群样本中,HCV抗体阳性498份,核酸检测呈阳性反应的样本390份,通过HCV基因分型试剂盒确定型别的样本311份,经CORE区测序确定型别的样本70份,有9份未成功扩增出序列,共有381份样本确定了基因亚型。其主要基因亚型为3b、3a、1b、6n及6a五种型别,分别占38.6%(147/381)、20.5%(78/381)、18.6%(71/381)、10.8%(41/381)、8.7%(33/381);不同地区之间流行的HCV基因型别差异具有统计学意义(χ~2=282.23,P0.01);男性和女性之间主要基因型不太一致;各年龄段间HCV基因亚型分布的差异无统计学意义(χ~2=40.93,P0.05),但30~50岁感染者居多。不同基因型之间HCV核糖核酸含量的差异有统计学意义(F=5.85,P0.01)。结论静脉吸毒人群HCV感染者的基因亚型主要为1b、3a、3b、6a及6n,基因型分布具有地域性差异,并且病毒载量与基因型的分布具有相关关系。
[Abstract]:Objective to investigate the distribution of gene subtypes and influencing factors of hepatitis C virus (HCV) infection in intravenous drug users. Methods 828 samples of intravenous drug users were collected for primary screening of anti-HCV antibodies by enzyme linked immunosorbent assay (Elisa), and the reactive samples were tested with recombinant Western blotting (RIBA). The positive samples were tested for nucleic acid, and the positive samples were typed by HCV genotyping kit, then the HCV CORE region was amplified by nested polymerase chain reaction (NPCR) and sequenced. Results 498 samples were positive for HCV antibody, 390 samples were positive for nucleic acid detection, and 311 samples were identified by HCV genotyping kit. Of the 70 samples identified by CORE sequencing, 9 were not amplified successfully, and a total of 381 samples were identified as gene subtypes. There were significant differences in the genotypes of HCV between different regions (蠂 ~ 2282.23, P 0.01, 蠂 ~ 2 282.23, P 0.01), and there were significant differences between male and female in the major genotypes (P < 0.05), and 10.8% in 41 / 38 ~ (-1) / 10.8% ~ 8.7% ~ 3.7% respectively (蠂 ~ 2282.23 P 0.01), and there was a significant difference between the two groups (蠂 ~ 2282.23 P0.01), and there was a significant difference between the two groups (蠂 ~ (2) 282.23 P 0.01). There was no significant difference in the distribution of HCV gene subtypes among different age groups (蠂 ~ (2 +)) 40.93 (P 0.05), but the majority of patients aged 30 ~ 50 years were infected with HCV. There were significant differences in HCV ribonucleic acid content among different genotypes. The subtypes of the subtypes were mainly 1b ~ 3a ~ (3a) ~ (3b) ~ (6a) and ~ (6) n.The distribution of genotypes was different in different regions. There was a correlation between viral load and genotype distribution.
【作者单位】：中国疾病预防控制中心性病艾滋病预防控制中心参比室;
【分类号】：R512.91;R749.64

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