丝胶对2型糖尿病大鼠肝脏胰岛素受体底物-2表达的影响

发布时间：2018-02-28 12:28

  本文关键词： 丝胶 型糖尿病 肝脏 胰岛素受体底物- 胰岛素抵抗　出处：《中国老年学杂志》2016年19期 　论文类型：期刊论文

【摘要】：目的研究丝胶对2型糖尿病大鼠肝脏胰岛素受体底物(IRS)-2表达的影响。方法 36只雄性SD大鼠随机分为正常对照组、糖尿病模型组、丝胶治疗组,每组12只。采用链脲佐菌素连续腹腔注射法制作2型糖尿病大鼠模型。待模型成功建立后,丝胶治疗组大鼠给予丝胶(2.4 g·kg~(-1)·d~(-1))灌胃。SP免疫组织化学染色、免疫印迹法和RT-PCR检测大鼠肝脏组织IRS-2的表达。结果丝胶可明显提高糖尿病模型大鼠肝脏组织IRS-2阳性细胞数、IRS-2蛋白和mRNA的表达(P0.01)。结论丝胶可通过上调糖尿病大鼠肝脏IRS-2的表达,影响胰岛素的信号转导和改善胰岛素抵抗,起到降低血糖的作用。
[Abstract]:Objective to study the effect of sericin on the expression of insulin receptor substrate IRS-2 in liver of type 2 diabetic rats. Methods 36 male SD rats were randomly divided into normal control group, diabetic model group and sericin treatment group. The model of type 2 diabetic rats was established by continuous intraperitoneal injection of streptozotocin. After the successful establishment of the model, the rats in the sericin treatment group were given sericin 2.4 g 路kg ~ (-1) 路d ~ (-1)) by intragastric perfusion with immunohistochemical staining of SP. Results sericin could significantly increase the number of IRS-2 positive cells and the expression of IRS-2 protein and mRNA in the liver of diabetic rats. Conclusion sericin can up-regulate the expression of IRS-2 in the liver of diabetic rats. Affect insulin signal transduction and improve insulin resistance, play a role in reducing blood sugar.
【作者单位】： 承德医学院附属医院检验科;承德医学院人体解剖学教研室;
【基金】：国家自然科学基金项目(81441133) 河北省自然科学基金项目(H2013406096) 河北省高校重点发展学科“人体解剖与组织胚胎学”建设项目资助(2013年)
【分类号】：R587.1;R440
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