大麻素1型受体过表达在实验性自身免疫性脑脊髓炎小鼠中的作用

发布时间：2018-02-28 14:28

本文关键词： CB大麻酚受体实验性自身免疫性脑脊髓炎白介素类肿瘤坏死因子α　出处：《第二军医大学学报》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨大麻素1型受体(cannabinoid 1receptor,CB1R)过表达对实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)小鼠神经功能的影响及其作用机制。方法选取24只C57B/L6小鼠,采用MOG_(35-55)免疫诱导构建EAE小鼠模型,随机分为2组,一组采用质粒转染技术使小鼠脊髓组织CB1R过表达,另一组给予溶剂作为对照。观察对照组和CB1R过表达组EAE小鼠的神经功能缺损症状,采用蛋白质印迹法检测小鼠脊髓组织中CB1R蛋白的表达,采用酶联免疫吸附试验检测小鼠脊髓组织中细胞因子白介素(IL)-10、IL-17、IL-6、肿瘤坏死因子α(TNF-α)的浓度。结果免疫后第14、28天时CB1R过表达组EAE小鼠发生神经功能缺损的程度低于对照组(P0.05)。与对照组相比,CB1R过表达组EAE小鼠脊髓组织中CB1R蛋白的表达水平升高(P0.01),IL-10的浓度升高(P0.05),而IL-17、IL-6、TNF-α的浓度降低(P0.05,P0.01)。结论中枢神经细胞中CB1R的过表达能够延缓EAE的发生和发展,这种作用可能是通过免疫调节实现的。
[Abstract]:Objective to investigate the effect of cannabinoid 1 receptor CB1R (CB1R) overexpression on neural function and its mechanism in experimental autoimmune encephalomyelitis autoimmune encephalomyelitis (EAE) mice. The mice were randomly divided into two groups: one group was overexpressed by plasmid transfection technique, the other group was treated with solvent as control group. The neurological deficit symptoms of EAE mice in control group and CB1R overexpression group were observed. The expression of CB1R protein in mouse spinal cord was detected by Western blot. Enzyme linked immunosorbent assay (Elisa) was used to detect the concentration of IL-17 and TNF- 伪 in the spinal cord tissue of mice. Results the degree of neurological impairment in EAE mice with overexpression of CB1R on day 1428 after immunization was lower than that in control group. Compared with the control group, the expression level of CB1R protein in the spinal cord of EAE mice was increased, while the concentration of IL-10 was increased, while the concentration of IL-17, IL-6, TNF- 伪 was decreased. Conclusion the overexpression of CB1R in central nervous cells can delay the occurrence and development of EAE. This effect may be achieved through immune regulation.
【作者单位】：上海交通大学医学院附属新华医院神经内科;第二军医大学长征医院神经内科;
【基金】：国家自然科学青年基金(81200921)~~
【分类号】：R744.51

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