青蒿琥酯对高糖诱导的肾小球系膜细胞NF-κB、MCP-1的研究

发布时间：2018-02-28 20:21

本文关键词： 糖尿病肾病肾小球系膜细胞 NF-κB MCP-1 青蒿琥酯　出处：《桂林医学院》2017年硕士论文　论文类型：学位论文

【摘要】：目的:通过观察青蒿琥酯(Art)对高糖培养条件下大鼠肾小球系膜细胞(HBZY-1)的增殖、凋亡及核因子(NF)-κB、单核细胞趋化蛋白-1(MCP-1)表达的变化,探讨Art对糖尿病肾病保护性作用的炎性机制。方法:传代培养大鼠肾小球系膜细胞HBZY-1,分为低糖(5.6 mmol/L葡萄糖)组、高糖(25 mmol/L葡萄糖)组、高糖+不同浓度青蒿琥酯(10、20、30 mg/L)组。48 h后采用四甲基偶氮唑盐微量比色(MTT)法观察细胞增殖能力改变;流式细胞仪Annexin V-FITC/PI双染法检测细胞凋亡指数;酶联免疫吸附(ELISA)法检测细胞上清液NF-κB、MCP-1的含量。结果:(1)经高糖处理48 h后,HBZY-1细胞较低糖组增殖能力及凋亡率均增加,细胞上清液NF-κB、MCP-1浓度升高。(2)经Art干预后,与高糖组比较,HBZY-1细胞增殖抑制,细胞凋亡率增加,细胞上清液NF-κB、MCP-1浓度降低,其干预作用呈现剂量依赖性。结论:Art能剂量依赖性地逆转高糖的作用。Art对高糖诱导下肾小球系膜细胞的增殖与凋亡,炎症因子的作用,可能为临床上糖尿病肾病提供新的治疗策略。
[Abstract]:Aim: to observe the effects of artesunate on the proliferation, apoptosis and expression of nuclear factor NF-kappa B, monocyte chemoattractant protein-1 (MCP-1) in rat glomerular Mesangial cells in high glucose culture. To investigate the inflammatory mechanism of protective effect of Art on diabetic nephropathy methods: cultured rat glomerular Mesangial cells (HBZY-1) were divided into low glucose group (5.6 mmol/L glucose) and high glucose 25 mmol/L group. After 48 h, the cell proliferation ability was observed by tetramethylazolium microcolorimetry, and the apoptosis index was detected by flow cytometry (Annexin V-FITC / Pi double staining). The content of MCP-1 in supernatant of HBZY-1 cells was detected by Elisa. Results: the proliferation ability and apoptosis rate of HBZY-1 cells were increased after 48 h of high glucose treatment, and the concentration of NF- 魏 BnMCP-1 in supernatant of HBZY-1 cells was higher than that of low glucose group. The concentration of NF- 魏 BnMCP-1 in supernatant of HBZY-1 cells was increased after treated with Art for 48 h. Compared with high glucose group, the proliferation of HBZY-1 cells was inhibited, the cell apoptosis rate was increased, and the concentration of NF- 魏 B-1 MCP-1 in the supernatant was decreased. ConclusionThe effect of high glucose on proliferation and apoptosis of glomerular Mesangial cells induced by high glucose was reversed in a dose-dependent manner, and the effect of inflammatory cytokines on the proliferation and apoptosis of glomerular Mesangial cells induced by high glucose was reversed in a dose-dependent manner. May provide a new treatment strategy for clinical diabetic nephropathy.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R587.2;R692.9

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