胰高血糖素样肽-1对高糖下兔胸主动脉内皮细胞HSP70表达的影响

发布时间：2018-03-02 21:41

本文选题：GLP-1　切入点：高糖　出处：《福建医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的探讨胰高血糖素样肽(glucagon-like peptide-1,GLP-1)对高糖环境诱导兔胸主动脉内皮细胞增殖、凋亡和热休克蛋白(Heat shock Protein,HSP)70表达的影响。方法胶原酶消化法培养兔胸主动脉内皮细胞,加入不同处理因素进行分组,TUNEL法测定内皮细胞的凋亡,BRDU法测定内皮细胞的增殖,Western blot法测定内皮细胞HSP70的表达水平。结果与对照组相比,高糖(33 mmol/L)培养24 h后兔胸主动脉内皮细胞增殖下降、细胞凋亡率增加、细胞HSP70的表达水平增加(P0.05);高糖条件下加入GLP-1(10-8M)共培养,与高糖组相比较,内皮细胞增殖增加、细胞凋亡率减少、细胞HSP70表达水平减少(P0.05);加入GLP-1前,先用GLP-1受体抑制剂(10-7M)预处理,与高糖组相比,内皮细胞的增殖、凋亡与HSP70表达则无明显区别(P㧐0.05)。结论GLP-1通过GLP-1受体依赖途径促进高糖条件下兔胸主动脉内皮细胞增殖,抑制细胞凋亡,减低高糖HSP70表达水平;GLP-1对延缓糖尿病血管病变可能与其降低高糖下内皮细胞HSP70表达水平有关。
[Abstract]:Objective to investigate the effects of glucagon-like peptide-1 (GLP-1) on the proliferation, apoptosis and heat shock protein (HSP70) expression of rabbit thoracic aortic endothelial cells induced by high glucose. Methods the rabbit aortic endothelial cells were cultured by collagenase digestion. The apoptosis of endothelial cells was determined by Tunel method and the proliferation of endothelial cells was determined by BRDU method. The expression of HSP70 in endothelial cells was determined by Western blot assay. Results compared with the control group, the expression of HSP70 in endothelial cells was determined by TUNEL-mediated dUTP Nick end labeling (Tunel). After high glucose 33 mmol / L culture for 24 h, the proliferation of rabbit thoracic aorta endothelial cells decreased, the apoptosis rate increased, and the expression level of HSP70 increased in rabbit thoracic aorta, and GLP-1 + 10-8 M-1 was added in high glucose condition. Compared with high glucose group, the proliferation of endothelial cells increased. The rate of apoptosis was decreased and the expression of HSP70 was decreased (P 0.05). Before the addition of GLP-1, pretreatment with GLP-1 receptor inhibitor 10 ~ (-7) M, compared with high glucose group, the proliferation of endothelial cells, apoptosis and HSP70 expression were not significantly different. Conclusion GLP-1 can promote the proliferation of rabbit thoracic aortic endothelial cells and inhibit apoptosis through GLP-1 receptor dependent pathway. To reduce the level of high glucose HSP70 expression and the effect of GLP-1 on delaying diabetic vascular disease may be related to the decrease of HSP70 expression in endothelial cells under high glucose.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.2

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