PDX-1基因转染人脂肪间充质干细胞向胰岛样细胞分化及移植治疗1型糖尿病

发布时间：2018-03-04 03:15

本文选题：脂肪组织　切入点：间质干细胞移植　出处：《中国组织工程研究》2017年13期 　论文类型：期刊论文

【摘要】：背景:近年研究发现脂肪间充质干细胞不仅具有多向分化潜能,在血糖平衡及激素产生方面也具有重要作用。目的:观察PDX-1基因转染脂肪间充质干细胞向胰岛样细胞的分化能力及移植后对糖尿病大鼠的治疗效果。方法:采用腺病毒转染方法将PDX-1基因转入人脂肪间充质干细胞,双硫腙染色鉴定细胞分化情况,ELISA法检测细胞胰岛素分泌水平。从20只雄性SD大鼠中随机取4只作为对照组,注射生理盐水,其余16只按65 mg/kg的剂量注射链脲霉素建立糖尿病模型,再随机等分为移植组和糖尿病组,移植组大鼠尾静脉注射PDX-1基因转染脂肪间充质干细胞。结果与结论:(1)转染后15 d,转染组胰岛素阳性细胞数量、胰岛素分泌量均高于未转染组(P0.05);(2)移植组大鼠空腹血糖水平显著下降(P0.05),体质量明显增加(P0.05),而糖尿病大鼠空腹血糖维持在较高水平,且体质量显著下降;(3)结果提示,导入PDX-1基因对人脂肪间充质干细胞分化为胰岛样细胞具有促进作用,PDX-1转染脂肪间充质干细胞移植能有效治疗大鼠糖尿病,但具体机制尚待进一步研究。
[Abstract]:Background: recent studies have found that adipose mesenchymal stem cells not only have the potential of multiple differentiation, Objective: to observe the differentiation ability of adipose mesenchymal stem cells into islet cells transfected with PDX-1 gene and the therapeutic effect of transplantation on diabetic rats. PDX-1 gene was transfected into human adipose mesenchymal stem cells by adenovirus transfection. Dithizone staining was used to identify the differentiation of cells and Elisa was used to detect the insulin secretion of the cells. Four male SD rats were randomly selected as control group and injected with normal saline. The other 16 rats were injected with streptozotocin for 65 mg/kg to establish diabetic model, and were randomly divided into transplantation group and diabetes group. The adipose mesenchymal stem cells were transfected with PDX-1 gene in tail vein of transplantation group. Results and conclusion the number of insulin-positive cells in transfection group was 15 days after transfection. Insulin secretion was significantly higher than that in the untransfected group (P 0.05) the fasting blood glucose level in the transplantation group was significantly lower than that in the control group, and the body mass increased significantly, while the fasting blood glucose level in the diabetic rats was maintained at a higher level, and the body mass decreased significantly. Transfection of PDX-1 gene into human adipose mesenchymal stem cells can promote the differentiation of adipose mesenchymal stem cells into islet like cells. PDX-1 transfected adipose mesenchymal stem cells transplantation can effectively treat diabetic rats, but the mechanism remains to be further studied.
【作者单位】：青岛市市立医院肝胆外科;青岛大学医学院;
【基金】：山东省医药卫生科技发展计划(2014WS0217)~~
【分类号】：R587.1

【相似文献】