糖尿病兔心肌缺血早期心肌细胞超微结构和心肌组织中肿瘤坏死因子α表达水平及其意义

发布时间：2018-03-04 11:20

本文选题：心肌缺血　切入点：糖尿病　出处：《吉林大学学报(医学版)》2017年03期 　论文类型：期刊论文

【摘要】：目的:探讨糖尿病兔心肌缺血早期心肌细胞超微结构和心肌组织中肿瘤坏死因子α(TNF-α)表达水平的变化及其作用机制,为研究糖尿病心肌缺血损伤发生机制提供理论依据。方法:选择42只健康雄性新西兰大白兔,其中26只采用四氧嘧啶耳缘静脉注射方法建立糖尿病模型,将建模成功的22只白兔采用开胸部分结扎冠状动脉左前降支方法建立糖尿病心肌缺血模型。将建模成功的16只糖尿病心肌缺血模型兔分为糖尿病心肌缺血7d组和28d组(n=8),其余16只白兔按照相应时间点分为假手术对照7d和28d组(n=8),只穿线不结扎。透射电镜下观察各组兔心肌细胞超微结构,TUNEL法测定各组兔缺血心肌组织中心肌细胞凋亡指数(AI),实时荧光定量PCR法测定各组兔缺血心肌组织中TNF-α表达水平。结果:假手术对照组兔心肌细胞核较大,核内染色质分布均匀,肌原纤维排列规则,肌节整齐,肌原纤维间线粒体丰富,结构清晰,而糖尿病心肌缺血组兔早期心肌细胞核不规则,肌原纤维结构松散,部分肌原纤维断裂,心肌细胞间线粒体增生明显。与假手术对照组比较,糖尿病兔心肌缺血7d和28d组缺血心肌细胞AI和TNF-α表达水平均明显升高(P0.05),且心肌缺血7d组高于心肌缺血28d组(P0.05)。结论:糖尿病兔心肌缺血早期发生心肌细胞超微结构破坏,心肌细胞凋亡增加;且TNF-α表达水平的升高参与了糖尿病心肌缺血损伤的发生。
[Abstract]:Objective: to investigate the changes of myocardial ultrastructure and the expression of tumor necrosis factor- 伪 (TNF- 伪) in myocardium of diabetic rabbits at the early stage of myocardial ischemia. Methods: 42 healthy male New Zealand white rabbits were selected to establish diabetic model by alloxan auricular vein injection. The diabetic myocardial ischemia model was established in 22 successful rabbits by partial ligation of left anterior descending coronary artery. Sixteen successful diabetic myocardial ischemia models were divided into diabetic myocardial ischemia group for 7 days and diabetic myocardial ischemia model for 7 days. The other 16 rabbits were divided into sham operation control group (7d) and 28d group (28 d) without ligation. The ultrastructure of myocardial cells in each group was observed by transmission electron microscope (TEM) and Tunel method was used to measure the myocardial size in ischemic myocardium. The expression of TNF- 伪 in ischemic myocardium was measured by real-time fluorescence quantitative PCR. Results: the myocardial nucleus of rabbits in sham operation control group was larger than that in control group. The distribution of chromatin in the nucleus was uniform, the myofibrils were arranged regularly, the myofibers were regular, the mitochondria were abundant and the structure was clear, while in the diabetic myocardial ischemia group, the nucleus of myocardium was irregular and the structure of myofibrils was loose. Some myofibrils were broken and mitochondria proliferated obviously in myocardial cells. Compared with the sham-operated control group, The expression level of AI and TNF- 伪 in myocardial ischemia group was significantly higher than that in myocardial ischemia group on 7 and 28 days after myocardial ischemia. Conclusion: myocardial ultrastructure damage occurred in early stage of myocardial ischemia in diabetic rabbits, and the expression level of TNF- 伪 in myocardial ischemia group was significantly higher than that in myocardial ischemia group on 7 day and 28 days after myocardial ischemia. Increased myocardial apoptosis and increased expression of TNF- 伪 were involved in diabetic myocardial ischemia injury.
【作者单位】：西安交通大学第一附属医院内分泌科;
【基金】：国家自然科学基金资助课题(81200098) 陕西省科技厅科技攻关项目资助课题(2014K11-03-04-06)
【分类号】：R542.2;R587.2

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