HIV抗体检测替代策略在全人群中推广应用的可行性研究

发布时间：2018-03-06 16:45

本文选题：艾滋病病毒　切入点：抗体检测　出处：《中国艾滋病性病》2017年01期 　论文类型：期刊论文

【摘要】：目的评价艾滋病病毒(HIV)抗体检测替代策略在各类人群中检测HIV抗体的可靠性,探讨HIV抗体替代策略在全人群中推广的可行性。方法对1 040份筛查阳性的样本,用6种不同的快速试剂、1种酶联免疫吸附试验(ELISA)试剂及蛋白印迹法(WB)同时进行检测,对WB不确定样本做病毒载量(VL)检测,对检测结果进行比较。结果 1 040份筛查阳性样本中,WB检测结果为阳性的有1 007份,当2种快速试剂检测结果为阳性,ELISA试剂检测结果 S/CO≥1时,一致性为100%;WB检测结果为不确定,且VL检测结果为阳性的有20份,当2种快速试剂检测结果为阳性,ELISA试剂检测结果 S/CO≥6时,一致性为100%;以上结果在各类人群中均一致。结论当2种快速试剂检测结果为阳性,且ELISA试剂检测结果 S/CO≥6时,与WB或VL检测结果一致性达到100%,可以替代WB确证检测。在保证检测试剂质量的情况下,将HIV抗体检测替代策略向全人群推广应用是可行的。
[Abstract]:Objective to evaluate the AIDS virus (HIV) antibody detection of alternative strategies for reliability of HIV antibodies in various populations, feasibility of promoting HIV antibody alternative strategy in the whole population. Methods 1040 cases of screening positive samples, using 6 different fast reagent 1, enzyme-linked immunosorbent assay (ELISA) kit and Western blot (WB) were detected at the same time, WB of uncertain sample viral load (VL) detection, to compare the test results. The results of 1040 screening positive samples, WB positive test results is 1007, when the 2 fast reagent test results were positive, the detection results of ELISA reagent S/CO more than 1, the consistency is 100%; WB detection result is uncertain, and the VL test results positive for 20, when 2 rapid test positive test results, test results of ELISA when S/CO is greater than 6, the consistency is 100%; the results in all kinds of people are Consistent. Conclusion when 2 rapid test positive test results, and ELISA reagent test results when S/CO is greater than 6, and the WB or VL test results consistency reached 100%, can replace the WB confirmed detection. Under the guarantee of the quality of the kit, it is feasible to alternative strategies for HIV antibody detection of wide application to the crowd.

【作者单位】：云南省疾病预防控制中心;昆明市疾病预防控制中心;
【分类号】：R512.91;R446.6

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