户尘螨Derp 2 T细胞表位融合肽对哮喘小鼠STAT6信号通路的影响

发布时间：2018-03-09 13:35

本文选题：户尘螨　切入点：特异性免疫治疗　出处：《中国寄生虫学与寄生虫病杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的通过户尘螨(Dermatophagoides pteronyssinus)2类变应原Der p 2 T细胞表位融合肽对哮喘小鼠的特异性免疫治疗,探讨信号转导因子和转录活化因子6(signal transducer and activator of transcription factor 6,STAT6)的变化及其特异性免疫治疗的作用机制。方法 30只小鼠用随机数表法分为哮喘组、Der p 2 T细胞表位融合肽免疫治疗组(简称免疫治疗组)和阴性对照组(PBS组),每组10只。哮喘组和免疫治疗组分别于第0、7、14天经小鼠腹腔注射100μl致敏液[含100μg/ml Der p2和2%Al(OH)3的PBS液],PBS组注射等量PBS液[含2%Al(OH)3]。哮喘组和免疫治疗组自第21天起,雾化吸入0.5μg/ml Der p 2致敏液,1次/d×30 min,连续7 d;PBS组雾化吸入等量PBS液。免疫治疗组在第25~27天雾化前30 min,经腹腔注射100μg/ml Der p 2 T细胞表位融合肽200μl,PBS组和哮喘组注射等量PBS液。末次雾化吸入24 h后处死小鼠,收集每组小鼠的支气管肺泡灌洗液(BALF),用ELISA检测BALF中白细胞介素-4(IL-4)、IL-13、γ干扰素(IFN-γ)水平。取肺组织提取肺组织全蛋白,蛋白质印记(Western blotting)检测肺组织全蛋白中STAT6和磷酸化STAT6(p-STAT6)的表达情况。组间样本的均数比较采用单因素方差分析。结果免疫治疗组小鼠的IFN-γ水平为(234.40±24.46)pg/ml,高于哮喘组的(155.80±20.53)pg/ml(P0.01);免疫治疗组小鼠的IL-4和IL-13水平分别为(30.00±5.50)和(174.50±25.99)pg/ml,均低于哮喘组小鼠的(53.28±8.26)和(308.10±28.32)pg/ml(P0.01)。免疫治疗组小鼠STAT6、p-STAT6的相对表达量分别为0.803±0.221和0.966±0.323,均低于哮喘组的1.669±0.296和1.735±0.298(P0.01)。结论 Der p 2 T细胞表位融合肽可能通过抑制STAT6治疗哮喘组小鼠。
[Abstract]:Objective to study the specific immunotherapy of asthmatic mice with Dermatophagoides pteronyssinus)2 Der p2 T cell epitope fusion peptide. To investigate the changes of signal transducer and activator of transcription factor 6T 6 and the mechanism of specific immunotherapy. Methods Thirty mice were randomly divided into asthmatic group with Der p2 T cell epitope fusion peptide. There were 10 rats in each group in immunotherapy group and 10 in negative control group. The asthmatic group and immunotherapy group were injected 100 渭 l sensitized solution [100 渭 g / ml Der p2 and 2AlOHN3 PBS solution] by intraperitoneal injection on the 7th and 14th day, respectively, in the asthmatic group and the immunotherapy group. PBS solution [2] .Asthma group and immunotherapy group from the 21st day, 0. 5 渭 g / ml Der p2 sensitizer was inhaled once per day for 30 minutes, and the same amount of PBS was inhaled continuously for 7 days. In the immunotherapy group, 100 渭 g / ml Der p2 T cell epitope fusion peptide was injected intraperitoneally 30 minutes before atomization on the 25th day, and the same amount was injected into the asthma group and 100 渭 g / ml Der p2 T cell epitope fusion peptide. The mice were killed 24 hours after the last inhalation of PBS solution. The bronchoalveolar lavage fluid (BALFN) was collected from each group of mice. The levels of IL-4, IL-4, IL-13, IFN- 纬 in BALF were detected by ELISA. The expression of STAT6 and phosphorylated STAT6 p-STAT6) in lung tissue was detected by Western blotting. The results showed that the level of IFN- 纬 in immunotherapy group was 234.40 卤24.46 渭 g / ml, higher than that in asthma group (155.80 卤20.53pgml-1). The levels of IL-4 and IL-13 in the immunotherapy group were 30.00 卤5.50) and 174.50 卤25.99pg / ml respectively, which were lower than those in the asthmatic group (53.28 卤8.26) and 308.10 卤28.32pg / ml / ml respectively. The relative expressions of STAT6p-STAT6 in immunotherapy group were 0.803 卤0.221 and 0.966 卤0.323respectively, which were lower than those in asthmatic group (1.669 卤0.296 and 1.735 卤0.298p 0.01g / ml). Conclusion the relative expression of STAT6p-STAT6 in immunotherapy group is 0.803 卤0.221 and 0.966 卤0.3233.Conclusion the relative expression of STAT6p-STAT6 in immunotherapy group is 0.803 卤0.221 and 0.966 卤0.323, which is lower than that in asthma group. The fusion peptide may inhibit the treatment of asthmatic mice by inhibiting STAT6.
【作者单位】：皖南医学院活性生物大分子研究安徽省重点实验室;安徽师范大学生命科学学院;
【基金】：国家自然科学基金(No.81270091,30872367) 安徽省高校自然科学研究重点项目(No.KJ2016A736) 安徽省自然科学基金(No.070413088)~~
【分类号】：R562.25

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