对讲机使用产生的电磁暴露对Wif-1基因甲基化水平及血液中淋巴细胞构成的影响

发布时间：2018-03-16 07:24

本文选题：电磁暴露　切入点：表观遗传　出处：《高电压技术》2017年02期 　论文类型：期刊论文

【摘要】：有研究提示电磁暴露可能是白血病发病的潜在危险因素。为探讨对讲机产生的电磁暴露对Wif-1基因甲基化水平及血液中白细胞构成的影响,采用1:1配对设计,选择年龄匹配的60名男性作为研究对象,最终纳入分析的为对讲机使用组(暴露组)29名,非使用组(对照组)28名。采用自行设计的流行病学调查问卷收集研究对象的一般人口学特征及职业暴露情况等。采用美国SEQUENOM Mass Array?Epi TYPER技术平台检测Wif-1基因的甲基化水平。采用配对t检验和多因素混合效应模型分析对讲机使用和Wif-1基因甲基化水平,以及Wif-1基因甲基化水平和血液中白细胞构成的关系。研究结果显示:在Wif-1基因启动子区域的32个CpG位点中,7个位点(CpG7、CpG8、CpG12、CpG16、CpG21、CpG28和CpG31)的甲基化水平暴露组显著高于对照组;电磁暴露与Wif-1基因平均甲基化水平呈正相关(偏回归系数β=2.19,概率P=0.026)。而Wif-1基因甲基化水平与淋巴细胞百分比呈正相关(β=0.633,P=0.020),与嗜酸性粒细胞百分比呈负相关(β=-0.151,P=0.016)。结果表明电磁暴露可能通过调节Wif-1基因甲基化水平而影响血液中淋巴细胞的构成,因而遗传的改变可能是电磁暴露致癌的重要机制,但是还需要更大样本量的研究来进一步确认。
[Abstract]:Some studies have suggested that electromagnetic exposure may be a potential risk factor for leukemia. In order to investigate the effect of electromagnetic exposure on the methylation level of Wif-1 gene and the leukocyte composition in blood, a 1: 1 pairing design was used. Sixty age-matched men were selected as study subjects, and the interphone use group (29 exposed group) was included in the analysis. A self-designed epidemiological questionnaire was used to collect the general demographic characteristics and occupational exposure of the subjects in the non-use group (control group, n = 28). The methylation level of Wif-1 gene was detected by Epi TYPER technique platform. The level of interphone use and Wif-1 gene methylation was analyzed by paired t test and multivariate mixed effect model. The relationship between the methylation level of Wif-1 gene and leukocyte composition in blood. The results showed that the methylation level of CpG7, CpG8, CpG12, CpG21, CpG21 and CpG31 in the 32 CpG loci of the promoter region of the Wif-1 gene were significantly higher in the exposed group than in the control group. There was a positive correlation between electromagnetic exposure and average methylation level of Wif-1 gene (partial regression coefficient 尾 2.19, probabilistic P = 0.026%), but a positive correlation between Wif-1 gene methylation level and lymphocyte percentage (尾 -0.633G, P < 0.020), and a negative correlation between Wif-1 gene methylation level and percentage of eosinophil (尾 -0.151). The results showed that the methylation level of Wif-1 gene was positively correlated with the percentage of lymphocytes. Electromagnetic exposure may affect the composition of lymphocytes in the blood by regulating the methylation level of the Wif-1 gene. Genetic changes may be an important mechanism of carcinogenesis in electromagnetic exposure, but more studies are needed to further confirm it.
【作者单位】：浙江大学医学院公共卫生学院流行病与卫生统计学学科;浙江大学医学院公共卫生学院生物电磁学重点研究实验室;浙江大学医学院附属第二医院神经外科;
【基金】：国家重点基础研究发展计划(973计划)(2011CB503706) 国家自然科学基金(81172624)~~
【分类号】：R594.8

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