肥胖对胰岛β细胞凋亡的影响

发布时间：2018-03-19 19:06

  本文选题：肥胖　切入点：凋亡　出处：《华中科技大学学报(医学版)》2017年06期 　论文类型：期刊论文

【摘要】：目的探讨肥胖对胰岛β细胞凋亡的影响及其可能的机制。方法取雄性SD大鼠,采用高脂饮食喂养的方法建立大鼠肥胖模型。取肥胖模型组(obese model group,OM组)和正常对照组(normal control group,NC组)大鼠各10只检测。测定空腹血糖(FBG)、总胆固醇(TC)、三酰甘油(TG)、游离脂肪酸(FFA)及血清胰岛素(FIns)的水平,计算胰岛素抵抗指数(HOMA-IR)。苏木精-伊红染色观察胰腺组织病理变化;脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测β细胞凋亡水平;Western blot检测细胞色素C(Cyt C)在线粒体、细胞质中的分布情况及活化的胱天蛋白酶-3(Caspase-3)的表达水平。结果与NC组比较,OM组大鼠FBG、TC、TG、FFA、FIns、HOMA-IR、凋亡指数、胞质Cyt C水平及活化的Caspase-3表达水平均明显升高;线粒体Cyt C水平明显降低(均P0.05);胰腺组织出现结构破坏、脂质变性等改变。结论肥胖通过线粒体途径增加胰岛β细胞的凋亡水平。
[Abstract]:Objective to investigate the effect of obesity on islet 尾 cell apoptosis and its possible mechanism. The obesity model of rats was established by high fat diet. The obesity model group (10 rats) and the normal control group group (NC group) were used to detect the fasting blood glucose (FBG), total cholesterol (TC), triacylglycerol (TG) and free lipid (FG) in the obese model group (n = 10) and the normal control group (n = 10). The levels of fatty acid FFAand serum insulin FIns, The insulin resistance index (HOMA-IRN), hematoxylin-eosin staining (HE staining), deoxyribonucleotidyl transferase-mediated Nick end labeling (DNT-dTTP) -mediated dUTP Nick end labeling (Tunel), and cytochrome C (Cyt C) in mitochondria were measured by blot, and the levels of apoptosis of 尾 cells were detected by Tunel Nick end labeling (Tunel). Results compared with the NC group, the expression of Caspase-3 in the cytoplasm and the expression of activated cystatin 3 were significantly higher in the OM group than in the control group. The apoptotic index, the level of cytoplasmic Cyt C and the expression of activated Caspase-3 were significantly higher in the OM group than in the control group. The level of mitochondrial Cyt C was significantly decreased (all P0.05%; pancreatic tissue structure was damaged, lipid denaturation and other changes). Conclusion Obesity can increase the apoptosis level of islet 尾 cells through mitochondrial pathway.
【作者单位】： 华中科技大学同济医学院附属梨园医院内分泌科;武汉市第一医院心血管内科;
【基金】：湖北省自然科学基金资助项目(No.2014CFB446)
【分类号】：R589.2
，

本文编号：1635598