建立雄性大鼠骨质疏松模型的方法学研究

发布时间：2018-03-21 02:01

  本文选题：男性骨质疏松症　切入点：D-半乳糖　出处：《中国骨质疏松杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨药物诱导与手术去势构建雄性大鼠骨质疏松模型的可行性,为进一步研究男性骨质疏松提供支持。方法雄性SD大鼠40只,随机分为4组(每组10只):空白对照组、手术组、给药组和共处理组。对照组未做处理;手术组行双侧睾丸切除方法;给药组行皮下注射D-半乳糖;共处理组同时行手术和皮下注射D-半乳糖。于干预前及干预后第8周测定体重,骨密度和骨矿盐含量,组织、脂肪、肌肉含量。结果术后第8周,三组实验组骨密度较对照组骨密度降低,共处理组和手术组骨密度分别下降12%和7%,统计学差异显著(P0.01);给药组骨密度下降2%,不存在统计学意义(P0.05);各组骨矿盐含量无差异(P0.05)。共处理组骨密度较手术组骨密度低5.6%,肌肉含量低19.8%,存在统计学差异(P0.05)。四组间体重无统计学差异(P0.05);三组实验组均低于对照组脂肪含量,统计学差异显著(P0.01),三组间脂肪含量无统计学差异(P0.05)。结论本研究手术去势法8周可以建立骨质疏松模型;去势法和皮下注射D-半乳糖联合使用8周建立骨质疏松模型骨密度下降更为明显;雄性动物骨质疏松后,脂肪含量减少。
[Abstract]:Objective to explore the feasibility of establishing osteoporosis model of male rats by drug induction and castration, and to provide support for further study on osteoporosis. Methods Forty male SD rats were randomly divided into 4 groups (10 rats in each group: blank control group). Operation group, administration group and co-treatment group. The control group was not treated, the operation group received bilateral orchiectomy, the drug group received subcutaneous injection of D-galactose, and the control group received subcutaneous injection of D-galactose. The body weight, bone mineral density, bone mineral salt content, tissue, fat and muscle content were measured before and 8 weeks after intervention. The BMD of the three experimental groups was lower than that of the control group. The BMD of co-treatment group and operation group decreased by 12% and 7, respectively, the difference was significant (P0.01), the BMD of administration group was decreased by 2%, there was no significant difference in bone mineral salt content in each group (P 0.05). The BMD of co-treatment group was lower than that of operation group (P < 0.05). 5.6The muscle content was 19.8%, there was statistical difference between the four groups (P 0.05), there was no significant difference in body weight among the four groups (P 0.05), and the fat content in the three groups was lower than that in the control group. There was no significant difference in fat content among the three groups (P 0.05). Conclusion Osteoporosis model can be established by ovariectomy for 8 weeks. The bone mineral density of 8 Zhou Jianli osteoporosis model decreased more obviously by castration and subcutaneous injection of D-galactose, and the fat content decreased after osteoporosis in male animals.
【作者单位】： 昆明医科大学第一附属医院;昆明学院;中国科学院昆明动物研究所;
【基金】：国家自然科学基金委-云南省人民政府联合基金项目:滇产彝药恒骨骨伤愈合剂促进骨质疏松骨形成机制的研究(U1502227) 国家自然科学基金项目:创建去卵巢树，

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