血管内皮祖细胞改善骨质疏松大鼠骨髓间充质干细胞的增殖及凋亡

发布时间：2018-03-25 00:20

本文选题：骨髓　切入点：间质干细胞　出处：《中国组织工程研究》2016年14期

【摘要】：背景:研究发现雌激素缺乏骨髓间充质干细胞的活性降低。目的:观察血管内皮祖细胞对去势骨髓间充质干细胞增殖及凋亡特性的影响。方法:1选用6周龄健康雌性SD大鼠行双侧卵巢切除术,建立骨质疏松症动物模型,假手术组仅切除卵巢周围等量脂肪组织;2采用密度梯度离心结合差速贴壁法分离、培养大鼠骨髓间充质干细胞;采用密度梯度离心法结合差速贴壁法利用促进内皮细胞生长的条件培养基诱导培养血管内皮祖细胞;3利用Transwell小室建立血管内皮祖细胞、去势骨髓间充质干细胞间接共培养体系,另设置正常骨髓间充质干细胞-骨髓间充质干细胞组,去势骨髓间充质干细胞-骨髓间充质干细胞组;4采用流式细胞术检测血管内皮祖细胞对去势骨髓间充质干细胞增殖和凋亡的影响。结果与结论:(1)间接共培养第3天,血管内皮祖细胞与去势骨髓间充质干细胞共培养组的S期细胞比例显著大于去势骨髓间充质干细胞组和正常骨髓间充质干细胞组,差异均有显著性意义(P0.05);(2)间接共培养第10天,血管内皮祖细胞与去势骨髓间充质干细胞共培养组骨髓间充质干细胞凋亡率显著小于去势骨髓间充质干细胞组和正常骨髓间充质干细胞组,差异均有显著性意义(P0.05);(3)通过血管内皮祖细胞和去势骨髓间充质干细胞间接共培养,发现血管内皮祖细胞可逆转体外去势骨髓间充质干细胞的增殖能力并且抑制其凋亡。t
[Abstract]:Background: it was found that the activity of bone marrow mesenchymal stem cells (MSCs) decreased in estrogen deficiency. Objective: to observe the effect of vascular endothelial progenitor cells on proliferation and apoptosis of ovariectomized bone marrow mesenchymal stem cells. Female SD rats underwent bilateral ovariectomy. The animal model of osteoporosis was established. In sham-operation group, bone marrow mesenchymal stem cells were cultured by density gradient centrifugation combined with differential adherent method. Vascular endothelial progenitor cells (VECs) were induced by density gradient centrifugation combined with differential adherent method in conditioned medium to promote the growth of endothelial cells. Vascular endothelial progenitor cells (VECs) were established by Transwell chamber, and bone marrow mesenchymal stem cells (BMSCs) were cocultured indirectly. In addition, the normal bone marrow mesenchymal stem cells (BMSCs) -bone marrow mesenchymal stem cells (BMSCs) group, The effects of vascular endothelial progenitor cells on proliferation and apoptosis of castrated bone marrow mesenchymal stem cells (BMSCs) were detected by flow cytometry. The proportion of S phase cells in vascular endothelial progenitor cells co-cultured with ovariectomized bone marrow mesenchymal stem cells was significantly higher than that in ovariectomized bone marrow mesenchymal stem cells group and normal bone marrow mesenchymal stem cells group. The apoptosis rate of bone marrow mesenchymal stem cells in the co-culture group of vascular endothelial progenitor cells and ovariectomized bone marrow mesenchymal stem cells was significantly lower than that in ovariectomized bone marrow mesenchymal stem cells group and normal bone marrow mesenchymal stem cell group. By indirect co-culture of vascular endothelial progenitor cells and ovariectomized bone marrow mesenchymal stem cells, it was found that vascular endothelial progenitor cells could reverse the proliferation of castrated bone marrow mesenchymal stem cells and inhibit their apoptosis.
【作者单位】：解放军第四军医大学口腔医院正畸科军事口腔医学国家重点实验室;解放军第四军医大学药学院天然药物学教研室;
【基金】：国家自然科学基金资助项目(81170982)~~
【分类号】：R580

【相似文献】