胰岛素样生长因子1受体基因rs2229765多态性与绝经后妇女骨质疏松的相关性

发布时间：2018-03-25 19:48

本文选题：骨质疏松　切入点：基因　出处：《中国组织工程研究》2017年12期

【摘要】：背景:胰岛素样生长因子1在骨细胞功能及骨代谢的调节中发挥重要作用。目前为止,胰岛素样生长因子1受体基因多态性与绝经后骨质疏松的相关性尚未见报道。目的:探讨胰岛素样生长因子1受体基因rs2229765单核苷酸多态性与绝经后妇女骨质疏松的相关性。方法:应用PCR-RFLP技术测定甘肃地区218例绝经后骨质疏松患者及270例骨量正常绝经妇女的胰岛素样生长因子1受体基因rs2229765多态性位点基因型。采用双能X射线吸收骨密度仪检测受试者腰椎、股骨颈以及前臂骨密度。应用ELISA测定血清胰岛素样生长因子1。结果与结论:(1)rs2229765多态性AA基因型(29%vs.17%,P=0.001)及等位基因A(51%vs.40%,P=0.000)在绝经后骨质疏松组中的分布频率显著高于骨量正常组;(2)与rs2229765基因型GG相比,基因型AA显著增加骨质疏松患病风险(OR=2.12,95%CI:1.27-3.54,P=0.004);(3)血清胰岛素样生长因子1分析结果显示,骨质疏松组中rs2229765 AA基因型(P=0.007)及GA基因型(P=0.016)妇女血清胰岛素样生长因子1水平显著低于GG基因型;(4)结果表明,胰岛素样生长因子1受体基因rs2229765多态性与绝经后妇女骨质疏松具有相关性,并可能改变血清胰岛素样生长因子1水平。
[Abstract]:Background: insulin-like growth factor 1 plays an important role in the regulation of osteocyte function and bone metabolism. The association between insulin-like growth factor 1 receptor gene polymorphism and postmenopausal osteoporosis has not been reported. Objective: to investigate the relationship between insulin-like growth factor 1 receptor gene rs2229765 single nucleotide polymorphism and postmenopausal osteoporosis. Methods: rs2229765 polymorphism genotype of insulin-like growth factor 1 receptor gene in 218 postmenopausal osteoporosis patients and 270 postmenopausal women with normal bone mass were determined by PCR-RFLP technique. The lumbar vertebrae were measured by X-ray absorptiometry. Bone mineral density of femoral neck and forearm. Serum insulin-like growth factor 1 was measured by ELISA. Results and conclusions the distribution frequency of the AA genotype 29vs.1751vs.40 in postmenopausal osteoporosis group was significantly higher than that of normal bone mass in postmenopausal osteoporosis group. Group 2 was compared with rs2229765 genotype GG. Genotype AA significantly increased the risk of osteoporosis. The serum levels of insulin-like growth factor 1 (IGF-1) in osteoporosis group were significantly lower than those in GG genotype P0. 007 (P 0. 007) and GA genotype P0. 016 (P 0. 016). The rs2229765 polymorphism of insulin-like growth factor 1 receptor gene is associated with osteoporosis in postmenopausal women and may change the level of serum insulin-like growth factor 1.
【作者单位】：甘肃中医药大学临床医学院;甘肃省人民医院普外科;甘肃省人民医院内分泌科;
【基金】：甘肃省卫生行业科研计划项目(GSWSKY-2014-01)~~
【分类号】：R580

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