3种植物活性成分联合改善2型糖尿病小鼠氧化应激效果研究

发布时间：2018-03-28 01:19

本文选题：型糖尿病　切入点：氧化应激　出处：《食品研究与开发》2017年08期

【摘要】：建立2型糖尿病小鼠模型,测定FBG、TG、TC、HDL、LDL、INS、FFA、MDA、ROS等,分析南瓜多糖、葛根黄酮、普洱茶褐素提取物单一及联合对糖脂代谢和氧化应激的作用效果及氧化应激与糖脂代谢的相关性。研究表明提取物单一及联合干预均对糖脂代谢和氧化应激指标有显著的改善(P0.05)。其中多糖与黄酮联合组对改善FFA、MDA的效果显著优于多糖组、黄酮组,对改善TG的效果显著优于多糖组(P0.05);多糖与茶褐素联合组较多糖组可显著降低TG、LDL、INS的水平(P0.05);茶褐素与黄酮联合组对TG、TC、LDL、FBG、INS、FFA的作用效果显著优于黄酮组,对TG的作用效果显著优于茶褐素组(P0.05)。利用主成分分析可知,联合与单一成分在改善糖、脂代谢及氧化应激指标上权重贡献率不尽相同,说明其作用机制的靶点和通路存在差异性。同时,通过线性相关分析法可知氧化应激与糖脂代谢具有极显著的正相关关系,提取物对T2DM小鼠糖脂代谢的改善与降低氧化应激水平有关。
[Abstract]:To establish a mouse model of type 2 diabetes mellitus, to determine the content of FBGG, TGG, HDL, LDLX, FFAA, MDAA, Ros, and to analyze the polysaccharides of pumpkin and the flavonoids of Pueraria lobata. The effect of Pu'er Tea Brown extract on glucose and lipid metabolism and oxidative stress and the relationship between oxidative stress and glucose lipid metabolism were studied. The effect of the combination of polysaccharide and flavonoids on the improvement of MDA in FFAA was significantly better than that in the polysaccharide group. The effect of flavonoids group on improving TG was significantly better than that of polysaccharide group (P 0.05), the level of TGN LDLins was significantly decreased in the combined group of polysaccharides and tea brownin, and the effect of the combination group of tea brownin and flavonoid on the TGCLDLN FBGG FFA was significantly better than that of the flavonoid group, and the effect of the combination group was significantly better than that of the flavonoid group, and the effect of the combination group was significantly better than that of the flavonoid group. The effect of TG on TG was significantly better than that on tea brownin group (P 0.05). The results of principal component analysis showed that the weight contribution rate of combined component and single component in improving glucose, lipid metabolism and oxidative stress index was not the same. At the same time, the linear correlation analysis showed that oxidative stress had a very significant positive correlation with glucose and lipid metabolism. The improvement of glucose and lipid metabolism in T2DM mice was related to the decrease of oxidative stress.
【作者单位】：天津农学院食品科学与生物工程学院;天津市农副产品深加工技术工程中心;
【基金】：天津市科技支撑计划项目(13ZCZDNC01800) 天津市农业科技成果转化项目(14ZXNZNC00036) 天津市科技特派员项目(15JCTPJC58500) 天津学院校内创新实验室建设项目(20171011)
【分类号】：R587.1

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