甘精胰岛素对初诊2型糖尿病Nrf2-Keap1-ARE信号通路的影响

发布时间：2018-03-28 04:24

本文选题：甘精胰岛素　切入点：型糖尿病　出处：《中国老年学杂志》2017年21期

【摘要】：目的探讨甘精胰岛素对初诊2型糖尿病(T2DM)患者核因子E2相关因子2-kelch样ECH连接蛋白1-抗氧化反应元件(Nrf2-Keap1-ARE)信号通路的影响。方法将50例糖尿病患者作为实验组,并且每晚睡前皮下注射甘精胰岛素;同时选取年龄、性别相匹配的健康者50人为对照组。收集所有人血液,检测血液生化指标:空腹血糖(FPG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、糖化血红蛋白(Hb A1c);酶联免疫吸附法(ELISA)检测甘精胰岛素治疗前后及对照组Nrf2、谷胱甘肽S-转移酶(GST)、超氧化物歧化酶(SOD)、血红素单加氧酶(HO)-1和醌氧化还原酶[NAD(P)H:NQO1]含量。结果与对照组相比,实验组治疗前FPG、TC、TG、LDL-C和Hb A1c显著升高(P0.05),实验组治疗后较本组治疗前FPG、TC、TG、LDL-C和Hb A1c显著下降(P0.05);实验组治疗前SOD与GST水平明显低于对照组(P0.05),Nrf2、HO-1和NQO1显著高于对照组(P0.05);实验组治疗后较本组治疗前SOD与GST水平增加,Nrf2、HO-1与NQO1水平显著减少(P0.05)。结论糖尿病时Nrf2表达升高,其靶基因下游蛋白HO-1和NQO1也随之上升。甘精胰岛素通过抑制Nrf2-Keap1-ARE信号通路改善胰岛素抵抗,其机制可能与改善氧化应激状态有关,该信号通路可能成为治疗T2DM的潜在靶点。
[Abstract]:Objective to investigate the effect of insulin glycyrrhizin on the signal pathway of nuclear factor E2 related factor 2-kelch like ECH junction protein 1 (Nrf2-Keap1-ARE) in patients with newly diagnosed type 2 diabetes mellitus (T2DM). Methods 50 patients with diabetes mellitus were selected as experimental group. Insulin glargine was injected subcutaneously before bedtime every night, and 50 healthy people with matched age and sex were selected as control group. Blood was collected from all of them. Blood biochemical parameters were detected as follows: fasting blood glucose (FPGG), total cholesterol (TC), triglyceride (TGN), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), glycosylated hemoglobin (HbA1cN), and enzyme linked immunosorbent assay (Elisa) for the detection of glycerol before insulin therapy. The contents of Nrf2, glutathione S-transferase (GST), superoxide dismutase (SOD), heme monooxygenase (HOO-1) and quinone oxidoreductase (NAD(P)H:NQO1) in the control group were compared with those in the control group. Before treatment, the LDL-C and HbA1c levels of the experimental group were significantly higher than that of the control group (P 0.05). The LDL-C and HbA1c levels of the experimental group were significantly lower than those of the control group (P 0.05). The levels of SOD and GST in the experimental group were significantly lower than those in the control group (P 0.055H _ 2HO-1 and NQO1); in the experimental group, they were significantly higher than that in the control group (P 0.05); in the experimental group, the levels of SOD and GST were significantly lower than those in the control group (P 0.05). After treatment, the levels of SOD and GST increased significantly, and the levels of Nrf2HO-1 and NQO1 decreased significantly compared with those before treatment. Conclusion the expression of Nrf2 in diabetic patients is higher than that in patients with diabetes mellitus. Insulin glycosine ameliorates insulin resistance by inhibiting the Nrf2-Keap1-ARE signaling pathway, which may be related to the improvement of oxidative stress, which may become a potential target for the treatment of T2DM.
【作者单位】：中南大学湘雅二医院深圳医院;
【基金】：深圳市科技创新委基金课题(No.JCYJ20160428101710597)
【分类号】：R587.1

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