Apelin-13抑制高糖引起的肾小球系膜细胞炎症

发布时间：2018-03-29 23:10

本文选题：Apelin　切入点：糖尿病肾病　出处：《中国医院药学杂志》2016年03期

【摘要】：目的:在高糖刺激的肾小球系膜细胞MES13和HBZY-1中研究Apelin-13抑制高糖引起的细胞炎症。方法:在高糖诱导的MES13和HBZY-1细胞中给予不同剂量的Apelin-13,通过real time-PCR技术检测各实验组2种系膜细胞中Icam-1和Mcp-1基因的mRNA水平变化。结果:(1)与空白对照组细胞相比,高糖组MES13和HBZY-1细胞中Icam-1和Mcp-1基因的mRNA水平显著性上升(P0.05);给药Apelin-13(300 pmol·L-1)能显著性抑制高糖刺激的MES13和HBZY-1细胞中Icam-1和Mcp-1基因的mRNA水平(P0.05)。(2)与空白对照组细胞相比,通过免疫组化实验检测高糖组MES13和HBZY-1细胞中促炎蛋白p38的磷酸化水平上升(P0.05);给药Apelin-13(300 pmol·L-1)能显著性抑制高糖引起MES13和HBZY-1细胞中促炎症蛋白p38的磷酸化(P0.05)。结论:Apelin-13抑制高糖引起的MES13和HBZY-1细胞中p38的激活,从而抑制高糖所引起的炎症反应(Icam-1和Mcp-1基因水平)。
[Abstract]:Objective: To study the inhibition of Apelin-13 cells induced by high glucose in MES13 and HBZY-1 in mesangial cells stimulated by high glucose. The method given different dose in high glucose induced MES13 and HBZY-1 cells in Apelin-13, mRNA levels were detected by real time-PCR and Mcp-1 Icam-1 of each experimental group 2 mesangial cell genes. Results: (1) compared with the control group cells, Icam-1 and Mcp-1 genes of MES13 and HBZY-1 cells in high glucose group the level of mRNA was significantly increased (P0.05); administration of Apelin-13 (300 pmol L-1) Icam-1 and Mcp-1 can significantly inhibit glucose stimulated MES13 and HBZY-1 cells in gene level of mRNA (P0.05). (2) compared with the control group cells by immunohistochemical p38 assay of proinflammatory protein MES13 in high glucose group and HBZY-1 cells in the phosphorylation level increased (P0.05); administration of Apelin-13 (300 pmol L-1) could significantly inhibit high glucose induced The phosphorylation of proinflammatory protein p38 (P0.05) was observed in MES13 and HBZY-1 cells. Conclusion: Apelin-13 inhibits the activation of p38 in MES13 and HBZY-1 cells induced by high glucose, thereby inhibiting the inflammatory reaction induced by high glucose (Icam-1 and Mcp-1 gene level).

【作者单位】：武汉市第三医院;武汉沃尔药业有限公司;
【基金】：武汉市卫生计生委科研计划资助项目(编号:WX12B06) 湖北省自然科学基金面上项目(编号:2013CFB359) 湖北卫生和计划生育委员会科研计划资助项目(编号:WJ2015MB147)
【分类号】：R587.2

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