去卵巢大鼠骨质疏松模型的制备与评价

发布时间：2018-03-31 14:01

本文选题：去势　切入点：骨质疏松　出处：《中国骨质疏松杂志》2017年10期

【摘要】：目的制备并评价去势大鼠骨质疏松动物模型。方法选取SD大鼠(雌性,2-3月龄)64只。随机分为:模型组(50只)及正常组(14只),模型组采用摘除双侧卵巢结扎术制备骨质疏松雌性大鼠模型,俯卧位固定在大鼠固定板上,取背部切口,逐层切开,分离结扎输卵管,摘除双侧卵巢,依次缝合,碘伏消毒,预防感染(肌肉注射80万单位青霉素);正常组为对照组,自然饲养,不做任何处理。并采用大鼠阴道分泌物涂片法确定其动情周期以检测双侧卵巢摘除情况,自术后次日清晨开始,用无菌棉签获取各组大鼠阴道分泌物,涂片于载玻片,常规固定及风干后,进行瑞氏染色,连续5天。显微镜下观察阴道上皮角化细胞的形态变化。结果模型组以白细胞为主,有核上皮细胞少见,呈连续性动情间期;正常组呈规律的动情周期变化:动情前期→动情期→动情后期→动情间期。结论雌性大鼠去双侧卵巢可有效制备骨质疏松模型;采取观察阴道上皮角化细胞的检测方法是证明去势骨质疏松模型成功的标准。
[Abstract]:Objective to prepare and evaluate the osteoporosis animal model of ovariectomized rats. Methods Sixty-four SD rats (female, 2-3 months old) were randomly divided into two groups: model group (n = 50) and normal group (n = 14). The model group was prepared by bilateral ovarian ligation. Osteoporosis female rat model, The prone position was fixed on the fixed plate of the rat, the incision was taken from the back, the fallopian tube was separated and ligated layer by layer, the oviduct was removed, the bilateral ovaries were removed, followed by suture, iodophor disinfection, to prevent infection (800000 units of penicillin intramuscularly injected; control group: control group), The oestrous cycle was determined by smear method of vaginal secretion of rats to detect bilateral ovariectomies. From the morning after operation, vaginal secretions of each group were obtained with aseptic cotton swabs. The smears were stained by Rayleigh staining after glass slide fixation and air drying for 5 days. The morphological changes of epithelial keratinocytes in vaginal epithelium were observed under microscope. Results in model group, white blood cells were dominant, and nucleated epithelial cells were rare. Continuous estrous interval; regular estrous cycle in normal group: preestrus. 鈫扙strous period. 鈫扙strous period. 鈫扖onclusion Osteoporosis model can be established effectively by bilateral ovariectomy in female rats, and the method of observing vaginal epithelial keratinocytes is the standard to prove the success of ovariectomized model.
【作者单位】：江西中医药大学;
【基金】：国家自然基金项目(81360601)
【分类号】：R-332;R580

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