高效液相色谱-电化学检测法研究大蒜素对百草枯中毒小鼠脑组织中单胺类神经递质含量的影响

发布时间：2018-04-02 04:07

本文选题：高效液相色谱-电化学检测　切入点：百草枯　出处：《分析测试学报》2017年09期

【摘要】：建立了单胺类神经递质的高效液相色谱-电化学检测方法,探讨了大蒜素对百草枯中毒小鼠脑组织内的单胺类神经递质及其代谢产物的影响。健康ICR小鼠随机分为4组:正常对照(Con)组、大蒜素(DAS)组、百草枯染毒(PQ)组和大蒜素治疗(PQ+DAS)组,每组6只。PQ组灌胃PQ 35 mg/kg建立中毒模型,Con组和DAS组灌服同体积生理盐水。DAS组和PQ+DAS组小鼠于造模2、26、50 h后腹腔内各注射1次DAS(25 mg/kg),PQ组和正常组小鼠腹腔内注射等量无菌生理盐水。造模12 h后处死小鼠。收集小鼠脑组织样品并称重,处理后进样。色谱分析采用Acclaim 120 C18(150 mm×2.1 mm,3μm)色谱柱,以离子对缓冲盐溶液-甲醇(93∶7,体积比)为流动相,流速为0.27 m L/min;电化学检测器检测,工作电压为450 m V。结果表明,与正常组和DAS组比较,PQ组部分神经递质水平降低;DAS治疗后能够改变上述生化指标的变化;与PQ组相比,PQ+DAS组神经递质含量增高,差异均具有统计学意义(P0.05)。脑组织样品中5-HT、DA、5-HIAA、DOPAC和HVA低、中、高3水平的加标回收率均为79.8%~112.1%,相对标准偏差(RSD)小于10%。实验表明,大蒜素对百草枯中毒后小鼠体内含量降低的神经递质有明显的上调作用。
[Abstract]:A high performance liquid chromatography-electrochemical detection method for monoamine neurotransmitters was established. The effects of allicin on monoamine neurotransmitters and their metabolites in the brain of paraquat poisoned mice were investigated. Healthy ICR mice were randomly divided into 4 groups: normal control group (Cona), allicin group (Das). Paraquat (PQQ) group and allicin treatment group (PQ DAS) group, Six mice in each group were given PQ35 mg/kg by intragastric administration of PQ35 mg/kg in each group. The mice in the Con group and the DAS group were given the same volume of normal saline. DAS and PQ DAS for 50 hours after the model was made. There was no equal amount of intraperitoneal injection of DAS(25 mg / kg PQ and normal mice in the control group and once a DAS(25 mg / kg group. The mice were killed after 12 hours of modeling. The brain tissue samples were collected and weighed. After treatment, the chromatographic analysis was performed on Acclaim 120C18C18mm 脳 2.1mm ~ (3 渭 m) column. The mobile phase consisted of ion-pair buffer salt solution and methanol (93: 7, volume ratio) at a flow rate of 0.27 mL / min. The working voltage was 450 MV by electrochemical detector. Compared with the normal group and the DAS group, the level of some neurotransmitters in the PQ group decreased and the changes of the above biochemical indexes were changed after treatment, and the neurotransmitter content in the PQ DAS group was higher than that in the PQ group, and that in the PQ DAS group was higher than that in the PQ group. The difference was statistically significant (P 0.05). The recoveries of 5-HTDDAN 5-HIAADOPAC and HVA in brain tissue samples were all 79.8% and 112.1, respectively, and the relative standard deviation (RSD) was less than 10%. Allicin significantly up-regulated the neurotransmitters of paraquat poisoning in mice.
【作者单位】：河北医科大学公共卫生学院;
【基金】：国家自然科学基金资助项目(81573202)
【分类号】：O657;R595.4

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