富血小板血浆对糖尿病大鼠足溃疡的影响

发布时间：2018-04-05 23:28

  本文选题：糖尿病　切入点：皮肤溃疡　出处：《广西医科大学》2015年硕士论文

【摘要】：目的：探讨富血小板血浆(PRP)对高血糖状态下的糖尿病大鼠下肢皮肤溃疡愈合速度的影响,并初步研究富血小板血浆治疗糖尿病大鼠下肢皮肤溃疡的机制。方法：糖尿病大鼠形成8周后下肢皮肤溃疡造模,随机分为治疗组及对照组,创面分别给予富血小板凝胶或生理盐水包裹治疗。每天换药并测量溃疡面积,治疗后第5d、14d采集血清及伤口组织,用ELISA法测血清及组织中MMP-2、MMP-9、TIMP-1、AGEs含量,伤口组织送病理进行切片及HE染色观察病理改变。结果：伤口造模后第5天治疗组及对照组溃疡面积无差异,PRP治疗组组织中MMP-2、MMP-9含量均低于对照组,组织中TIMP-1含量高于对照组(P均0.05),HE染色新生血管数量多于对照组。治疗后第14天PRP治疗组溃疡面积小于对照组(P0.05), MMP-2、MMP-9及TIMP-1差异较前更明显(P0.01),HE染色PRP治疗组成纤维细胞数量多于对照组,炎症细胞浸润量低于对照组。2组间AGEs含量及血中MMP-2、MMP-9、TIMP-1含量在各时间段均无差异。结论：1.利用烫伤的方法可以成功的制造糖尿病下肢皮肤溃疡模型。2.富血小板血浆治疗可能通过降低组织中MMP-2、MMP-9及提高TIMP-1含量,改善MMP-9/TIMP-1比值,促进高血糖状态下的糖尿病大鼠下肢皮肤溃疡愈合。
[Abstract]:Objective: to investigate the effect of platelet-rich plasma (PRP) on the healing rate of skin ulcer of lower extremity in diabetic rats with hyperglycemia, and to study the mechanism of platelet rich plasma in treating skin ulcer of lower extremity of diabetic rats.Methods: diabetic rats were randomly divided into treatment group and control group after 8 weeks of formation of lower extremity skin ulcer. The wounds were treated with platelet-rich gel or normal saline respectively.The area of ulcer was measured every day. The serum and wound tissue were collected on the 5th day after treatment. The levels of MMP-2MMP 9 and TIMP-1AGEs in serum and tissue were measured by ELISA method. The pathological changes of wound tissue were observed by pathological section and HE staining.Results: there was no difference in ulcer area between the treatment group and the control group on the 5th day after wound modeling. The content of MMP-2 and MMP-9 in the treated group was lower than that in the control group, and the content of TIMP-1 in the tissue was higher than that in the control group. The number of neovascularization in the treated group was higher than that in the control group.On the 14th day after treatment, the area of ulcer in the PRP group was smaller than that in the control group (P 0.05), and the difference of MMP-2, MMP-9 and TIMP-1 was more obvious than that in the control group. The number of fibroblasts in the PRP treated with P0.01HE-HE staining was more than that in the control group.The amount of inflammatory cell infiltration was lower than that of control group. There was no difference in AGEs content and MMP-2, MMP-9 and TIMP-1 content in blood.Conclusion 1.The skin ulcer model of diabetic lower extremity can be made successfully by scalding method. 2.Platelet-rich plasma therapy may promote the healing of skin ulcer of lower extremities in diabetic rats with hyperglycemia by decreasing MMP-2 MMP-9 and increasing the content of TIMP-1 and improving the ratio of MMP-9/TIMP-1.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.2

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