2型糖尿病患者肠道菌群变化及意义

发布时间：2018-04-11 02:17

  本文选题：型糖尿病 + 肠道菌群　； 参考：《山东医药》2017年16期

【摘要】：目的探讨2型糖尿病(T2DM)患者肠道菌群变化的临床意义。方法选取8例T2DM患者与10例体检健康者,提取其粪便基因组DNA,应用菌属16S rRNA V4序列特异性引物,采用高通量测序技术分析肠道菌群多样性(以香农多样性指数表示)及种类(以丰度表示)。观察不同性别T2DM患者的肠道菌群种类差异,分析T2DM患者肠道优势菌属与空腹血糖(FPG)、餐后1 h血糖(1 h PG)和餐后2 h血糖(2 h PG)的关系。结果 T2DM患者与体检健康者香农多样性指数分别为3.70±0.29、3.30±0.20,二者比较P0.05。门水平下T2DM患者和体检健康者的肠道共同优势菌门为厚壁菌门、拟杆菌门、变形菌门和放线菌门,二者上述4种优势菌门丰度比较差异均无统计学意义(P均0.05)。属水平下T2DM患者和体检健康者的肠道共同优势菌属为拟杆菌属和毛螺杆菌科,体检健康者罗斯氏菌属丰度高于T2DM患者(P0.05)。女性T2DM患者拟杆菌属科丰度高于男性患者,假单胞菌属丰度低于男性患者(P均0.05)。T2DM患者柔嫩梭菌属丰度与2 h PG呈正相关(r=0.75,P0.05),其他肠道优势菌属与FPG、1 h PG、2 h PG均无关(P均0.05)。结论 T2DM患者肠道内存在菌群失调,并具有性别差异;柔嫩梭菌属与血糖水平有关,可为T2DM的治疗提供一种新的思路。
[Abstract]:Objective to investigate the clinical significance of intestinal flora changes in patients with type 2 diabetes mellitus (T 2 DM).Methods the fecal genomic DNA was extracted from 8 T2DM patients and 10 healthy controls. The 16s rRNA V4 sequence specific primers were used.The diversity of intestinal flora (expressed by Shannon's diversity index) and species (denoted by abundance) were analyzed by high-throughput sequencing technique.To observe the differences of intestinal flora in patients with T2DM of different genders, and to analyze the relationship between the predominant bacteria in the intestine of T2DM patients and fasting blood glucose (FPG), postprandial blood glucose (1 h) and postprandial blood glucose (2 h).Results the Shannon diversity index was 3.70 卤0.293.30 卤0.20 in T2DM patients and healthy controls, respectively (P 0.05).The common dominant bacteria phylum of intestinal tract in T2DM patients and healthy persons were phylum thunbergium, Bacteroides, Proteus and Actinomycetes. There was no significant difference in the abundance of the above four dominant bacteria between them (P < 0.05).The common intestinal dominant bacteria in T2DM patients and healthy individuals were Bacteroides and Helicobacteriaceae. The abundance of Roxella in healthy individuals was higher than that in T2DM patients (P 0.05).The abundance of Pseudomonas in female patients was higher than that in male patients, and the abundance of Clostridium tenella in patients with 0.05).T2DM was significantly lower than that in patients with 0.05).T2DM. There was a positive correlation between the abundance of Clostridium tenella and 2 h PG.Conclusion there is a maladjustment of intestinal flora in patients with T2DM, and there is a sex difference between them, and Clostridium tenella is related to blood glucose level, which may provide a new idea for the treatment of T2DM.
【作者单位】： 江苏大学附属医院;天益健康科学研究院;
【基金】：国家自然科学基金面上项目(81570721;81370965) 江苏省自然科学基金资助项目(BK20151331) “六大人才高峰”第十二批高层次人才项目(2015-WSN-006) 镇江市科技支撑-社会发展重点项目(SH2015028)
【分类号】：R587.1
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本文编号：1733990