原发性干燥综合征患者躯体症状及外周血NKT细胞调节机制研究

发布时间：2018-04-11 04:26

本文选题：原发性干燥综合 + 躯体症状　；参考：《北京协和医学院》2015年博士论文

【摘要】：目的评估原发性干燥综合征(primary Sjogren's syndrome, pSS)患者躯体症状分布情况及外周血NKT (Natural killer T-cells)细胞比例及IFN-y分泌变化；探索NKT细胞在pSS中的调节机制。方法通过躯体症状群量表-15(Patient-Health-Questionnaire-15, PHQ-15)对pSS患者和健康对照者的躯体症状发生及分布情况进行评估,并通过视觉模拟评分(visual analogue scale, VAS)了解患者生活质量受疾病影响程度。使用流式细胞仪检测pSS患者及健康对照者外周血NKT细胞比例及各亚群变化。使用a-半乳糖神经酰胺体外刺激扩增NKT细胞,评估患者NKT细胞增殖能力。对比血清、细胞培养液上清及NKT细胞胞内IFN-γ分泌变化。结果pSS患者躯体症状分布与健康人一致,但发生率高于健康人,躯体障碍等级也较健康人高。疲劳是pSS患者中最为常见的躯体症状。pSS患者外周血NKT细胞比例显著低于健康人,CD4+CD8-NKT亚群最显著。体外刺激扩增NKT细胞后IFN-γ分泌水平较健康人增强。结论pSS患者躯体症状发生率显著高于健康人,躯体障碍程度较健康人严重。pSS患者外周血NKT细胞比例降低。pSS患者NKT细胞IFN-γ合成分泌能力增强,Th1/Th2平衡受影响。
[Abstract]:Objective to evaluate the distribution of somatic symptoms in patients with primary Sjogren's syndrome (PSS), the percentage of NKT Natural killer T-cellsand the changes of IFN-y secretion, and to explore the regulatory mechanism of NKT cells in pSS.Methods the occurrence and distribution of somatic symptoms were evaluated by Patient-Health-Questionnaire-15 (PHQ-15) in patients with pSS and healthy controls.The percentage and subsets of NKT cells in peripheral blood of pSS patients and healthy controls were detected by flow cytometry.A-galactose-ceramide was used to stimulate and amplify NKT cells in vitro to evaluate the proliferative ability of NKT cells in patients.The changes of IFN- 纬 secretion in serum, supernatant of cell culture medium and NKT cells were compared.Results the distribution of somatic symptoms in patients with pSS was consistent with that in healthy persons, but the incidence rate was higher than that in healthy persons, and the grade of somatic disorders was higher than that of healthy people.Fatigue is the most common somatic symptom. The percentage of peripheral blood NKT cells in patients with PSS is significantly lower than that in healthy people.The level of IFN- 纬 secretion was increased after stimulation and amplification of NKT cells in vitro.Conclusion the incidence of somatic symptoms in patients with pSS is significantly higher than that in healthy persons. The degree of somatic disorders is significantly lower than that in patients with PSS. The proportion of NKT cells in peripheral blood of patients with PSS is lower. The ability of IFN- 纬 synthesis and secretion of NKT cells in patients with PSS is increased and the balance of Th1 / Th2 is affected.
【学位授予单位】：北京协和医学院
【学位级别】：博士
【学位授予年份】：2015
【分类号】：R593.2

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