间充质干细胞增强巨噬细胞功能治疗SLE实验研究

发布时间：2018-04-14 01:39

本文选题：间充质干细胞 + 系统性红斑狼疮　；参考：《免疫学杂志》2017年10期

【摘要】：目的从对巨噬细胞功能调控角度探讨脐带间充质干细胞(umbilical cord mesenchymal stem cells,UCMSC)对狼疮鼠的治疗机制,为临床用于治疗狼疮患者提供理论依据。方法 24周龄B6.MRL-Fas~(lpr)(B6/lpr)狼疮鼠随机分为空白对照组、UCMSC移植治疗组和成纤维样滑膜细胞(fibroblast like synoviocytes,FLS)移植对照组,同周龄雌性C57BL/6鼠作为正常对照。UCMSC和FLS组均给予1×10~6数量尾静脉输注,各组小鼠于28周龄处死。HE检测小鼠肾脏病理,评估疗效。分离纯化小鼠腹腔和肾脏巨噬细胞,无菌条件下取8周龄雌性C57BL/6小鼠脾脏,磁珠分选CD4~+T细胞,在抗CD3/28刺激下,将其以5∶1比例与小鼠巨噬细胞共培养4 d,CFSE法检测CD4~+T细胞增殖。2μm荧光微球与小鼠巨噬细胞共培养2 h,流式细胞术检测巨噬细胞吞噬活性。结果 (1)UCMSC组可显著改善狼疮鼠肾脏病理;(2)UCMSC移植治疗可显著提高狼疮鼠巨噬细胞的免疫调节能力,使其对CD4~+T细胞增殖的抑制能力显著增强;(3)UCMSC组狼疮鼠腹腔巨噬细胞的吞噬功能显著增强。结论 UCMSC移植治疗狼疮鼠可以改善肾脏病理损伤,UCMSC对巨噬细胞免疫调节和吞噬功能的调控是其发挥治疗作用的机制之一。
[Abstract]:Objective to investigate the therapeutic mechanism of umbilical cord mesenchymal stem cell (UCM) cord cord mesenchymal stem cells (UCM SCC) on lupus rats from the point of view of functional regulation of macrophages, and to provide a theoretical basis for clinical treatment of lupus.Methods Twenty-four week-old B6 MRL-FasLPRA B6 / lpr6 lupus mice were randomly divided into two groups: the control group treated with UCMSC transplantation and the control group transplanted with fibroblast like fibroblast like. The female C57BL/6 rats of the same age served as the normal control group. UCMSC and FLS groups were given 1 脳 10 6 caudal vein infusion.All the mice were killed at the age of 28 weeks. He was used to detect the renal pathology and evaluate the curative effect.Murine peritoneal and renal macrophages were isolated and purified. The spleen of 8-week-old female C57BL/6 mice was isolated under aseptic condition. CD4T cells were separated by magnetic beads.CD4T cells were co-cultured with mouse macrophages at 5:1 for 2 hours. The phagocytic activity of macrophages was detected by flow cytometry. The proliferation of CD4 ~ T cells was detected by fluorescence microspheres at 2 渭 m for 2 h, and the phagocytic activity of macrophages was detected by flow cytometry (FCM).Results UCMSC group could significantly improve the immunomodulation ability of macrophages in lupus rats.The inhibition of CD4T cell proliferation was significantly enhanced in UCMSC group. The phagocytic function of peritoneal macrophages was significantly enhanced in UCMSC group.Conclusion UCMSC transplantation can improve the regulation of macrophage immune regulation and phagocytosis by UCMSC, which is one of the therapeutic mechanisms of UCMSC in the treatment of lupus.
【作者单位】：南京大学医学院附属鼓楼医院风湿免疫科;
【基金】：国家自然科学青年科学基金(81401349) 南京市医学科技发展项目(YKK15073) 中央高校基金(021414340284)
【分类号】：R593.241

【相似文献】