Th17细胞及相关因子IL-17与绝经后骨质疏松的相关性研究

发布时间：2018-04-14 20:51

本文选题：辅助性T细胞 + 白细胞介素　；参考：《中国骨质疏松杂志》2016年05期

【摘要】：目的观察辅助性T细胞17(T helper cell 17,Th17)及及相关因子白细胞介素17(interleukin 17,IL17)在绝经后骨质疏松(Postmenopausal osteoporosis,PMOP)患者外周血及血清中的表达水平,并探讨IL-17细胞与绝经后骨质疏松的发病的关系。方法选取2011年7月~2014年7月在我院确诊的绝经后骨质疏松患者(60例)为研究组,选取同期来我院体检的绝经后骨量正常人群为对照组(60例),采用流式细胞术检测绝经后骨质疏松患者及骨质正常人外周血中Th17细胞水平,采用ELISA法检测两组人群外周血中IL-17、IL-6及TGF-β的蛋白水平;采用RT-PCR法检测两组人群外周血中转录因子T孤独核受体(retinoid-related orphan receptor gamma t,RORγT)的mRNA水平。通过Pearson相关分析绝经后骨质疏松患者中IL-17水平与骨密度(bone mineral density,BMD)以及血清钙可能存在的关系。结果绝经后骨质疏松患者外周血中Th17细胞比例为(2.17±0.41)%,显著高于健康对照组人群的(0.61±0.45)%,差异有统计学意义(P0.001);绝经后骨质疏松患者血清IL-17水平(31.51±10.09)pg/ml,显著高于对照组人群的(16.61±9.93)pg/ml,差异有统计学意义(P0.001);绝经后骨质疏松患者外周血RORγT mRNA的表达水平显著高于正常人群,差异有统计学意义(P0.001)。绝经后骨质正常对照组人群外周血血清中IL-6及TGF-β水平均显著低于绝经后骨质疏松患者,差异有统计学意义(t=8.99,P=0.000;t=4.71,P=0.000);此外,Pearson相关分析结果显示,绝经后骨质疏松患者血清中IL-17与BMD呈显著负相关(r=0.788,P0.05)。结论Th17细胞可能通过分泌IL-17促进绝经后骨质疏松的产生,IL-17可能作为治疗绝经后骨质疏松的重要靶点。
[Abstract]:Objective to investigate the expression of helper T cell 17T helper cell 17 (Th17) and its related factor 17(interleukin 17 (IL 17) in the peripheral blood and serum of postmenopausal osteoporosis patients, and to explore the relationship between IL-17 cells and postmenopausal osteoporosis.Methods 60 postmenopausal osteoporosis patients diagnosed in our hospital from July 2011 to July 2014 were selected as study group.Th17 cells in peripheral blood of postmenopausal osteoporosis patients and normal persons were measured by flow cytometry in 60 cases of normal postmenopausal bone mass in the same period of physical examination in our hospital, and 60 cases of normal postmenopausal bone mass were selected as control group.The levels of IL-17 IL-6 and TGF- 尾 in peripheral blood of two groups were detected by ELISA method, and the mRNA levels of transcription factor T lonely nuclear receptor (TLNF) in peripheral blood of two groups were detected by RT-PCR method.Pearson correlation was used to analyze the possible relationship between IL-17 level and bone mineral density (BMD) and serum calcium in postmenopausal osteoporosis patients.Results the percentage of Th17 cells in the peripheral blood of postmenopausal osteoporosis patients was 2.17 卤0.41g / ml, significantly higher than that of the healthy controls (0.61 卤0.45g / ml), and the serum IL-17 level of postmenopausal osteoporosis patients was 31.51 卤10.09ug / ml, which was significantly higher than that of the control group (16.61 卤9.93pg / ml).The expression of ROR 纬 T mRNA in postmenopausal osteoporosis patients was significantly higher than that in normal controls.The difference was statistically significant (P 0.001).The levels of IL-6 and TGF- 尾 in the peripheral blood of postmenopausal normal control group were significantly lower than those in postmenopausal osteoporosis patients (P < 0.05), and the difference was statistically significant (P < 0.05), and the results of Pearson correlation analysis showed that the serum levels of IL-6 and TGF- 尾 in the postmenopausal normal control group were significantly lower than those in the postmenopausal osteoporosis group (P < 0.05).There was a significant negative correlation between serum IL-17 and BMD in postmenopausal patients with osteoporosis.Conclusion Th17 cells may promote postmenopausal osteoporosis production by secreting IL-17. IL-17 may be an important target for the treatment of postmenopausal osteoporosis.
【作者单位】：上海市浦东新区浦南医院内分泌科;江苏省苏州大学医学院研究生院;上海市宝山区淞南社区服务中心;上海市第一人民医院;上海市浦东新区浦南医院脊柱外科;
【分类号】：R580

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