类风湿关节炎患者外周血肿瘤坏死因子受体相关因子1表达研究

发布时间：2018-04-16 01:00

本文选题：肿瘤坏死因子受体相关因子 + 关节炎　；参考：《中国实用内科杂志》2017年06期

【摘要】：目的检测类风湿关节炎(RA)患者血清肿瘤坏死因子受体相关因子1(TRAF1)水平,并分析其与RA疾病活动性关系,探讨其与CD40在RA发病机制中的共同作用。方法采集2015年7月至2015年12月苏州大学附属第一医院门诊及住院部70例初诊RA患者和50名健康体检者外周血,记录RA患者临床及实验室指标,酶联免疫吸附法(ELISA)检测TRAF1、CD40,并分析两者相关性。结果 RA患者TRAF1、CD40分别为(35.88±51.22)ng/L,(72.70±70.56)ng/L,与健康对照组[TRAF1和CD40分别为(12.51±8.59)ng/L和(31.54±27.11)ng/L]相比差异均有统计学意义(P0.05)。根据DAS28评分,将RA患者分为高、中、低活动期组,3组TRAF1分别为(13.05±4.29)ng/L、(29.84±42.48)ng/L和(58.84±66.90)ng/L,差异有统计学意义(F=6.382,P=0.003),且TRAF1与DAS28评分呈正相关(r=0.419,P0.001)。RA患者血清TRAF1与自身抗体RF、GPI呈正相关(r=0.365,P=0.001;r=0.767,P0.001)。RA患者血清TRAF1表达与CD40呈正相关(r=0.465,P0.001)。结论 TRAF1在RA患者明显增高,与RA疾病活动性、自身抗体和CD40呈正相关。
[Abstract]:Objective to detect the serum levels of tumor necrosis factor receptor associated factor (TNF- receptor 1) TRAF1 in patients with rheumatoid arthritis (RA), and to analyze the relationship between TNF- receptor 1 and the activity of RA, and to explore the role of TNF- receptor and CD40 in the pathogenesis of RA.Methods Peripheral blood samples were collected from 70 newly diagnosed RA patients and 50 healthy people in outpatient and inpatient departments of the first affiliated Hospital of Suzhou University from July 2015 to December 2015. The clinical and laboratory indexes of RA patients were recorded.TRAF1 CD40 was detected by Elisa and the correlation between them was analyzed.Results the CD40 of TRAF1 + ng / L in RA patients was 35.88 卤51.22 ng / L, respectively, which was 72.70 卤70.56 ng / L, which was significantly higher than that in healthy controls [TRAF1 and CD40 were 12.51 卤8.59)ng/L and 31.54 卤27.11)ng/L, respectively] (P 0.05).According to the DAS28 score, RA patients were classified as high, moderate,The TRAF1 of low activity group was 13.05 卤4.29 ng / L 29.84 卤42.48)ng/L and 58.84 卤66.90 ng / L respectively, the difference was statistically significant, and the TRAF1 and DAS28 score were positively correlated with the serum TRAF1 and the autoantibody RFGPI.Conclusion TRAF1 is significantly higher in RA patients, and is positively correlated with RA disease activity, autoantibodies and CD40.
【作者单位】：苏州大学附属第一医院风湿免疫科;苏州大学附属第一医院骨科;江苏省人民医院风湿免疫科;
【基金】：江苏省临床医学科技专项类风湿关节炎分子分型诊断研究(BL2013034) 苏州市科技局科技发展计划(SYSD2014078) 苏州大学青年教师自然科学基金(SDY2013A28)
【分类号】：R593.22

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