骨髓间充质干细胞移植在卵巢早衰小鼠卵巢及生育功能重建中的作用

发布时间：2018-04-17 06:01

本文选题：骨髓间充质干细胞 + 移植　；参考：《安徽医科大学学报》2017年11期

【摘要】：目的探讨骨髓间充质干细胞(BMSCs)移植在卵巢早衰小鼠卵巢及生育功能重建中的作用。方法一次性腹腔注射环磷酰胺和白消安建立卵巢早衰小鼠模型,将实验动物分为建模组、移植组和空白对照组。建模后14 d,将绿色荧光蛋白(GFP)转基因小鼠的BMSCs经尾静脉注射至移植组小鼠体内,取等量生理盐水经尾静脉注射至建模组和空白对照组小鼠体内。建模后14 d及BMSCs移植2个月检测各组血清卵泡刺激素(FSH)和雌二醇(E2)水平,BMSCs移植2个月观察BMSCs在卵巢组织的定位及卵巢颗粒细胞凋亡情况,记录各组超促排卵后的获卵数、受精率、卵裂率、囊胚形成率,以及与雄性小鼠合笼后妊娠及生育子代数量。结果BMSCs移植后2个月,移植组卵巢间质内可见大量绿色荧光细胞和标记的BMSCs,但并不表达FSH受体;FSH水平和颗粒细胞凋亡指数显著低于建模组(P0.05),E2水平及获卵数及受精率、卵裂率、囊胚形成率均显著高于建模组(P0.05),但各项指标仍显著低于空白对照组(P0.05);建模组无小鼠妊娠,移植组中2只小鼠妊娠,分别生育外观正常仔鼠4、2只。结论 BMSCs可定位于卵巢间质,显著降低卵巢颗粒细胞凋亡指数,改善卵巢内分泌功能,提高小鼠生育能力。
[Abstract]:Objective to investigate the role of bone marrow mesenchymal stem cells (BMSCs) transplantation in the reconstruction of ovarian and reproductive function in premature ovarian failure mice.Methods the mouse model of premature ovarian failure was established by intraperitoneal injection of cyclophosphamide and oxacol. The experimental animals were divided into three groups: model group, transplantation group and blank control group.On the 14th day after modeling, the BMSCs of GFP transgenic mice was injected into the mice of transplantation group through tail vein, and the same amount of normal saline was injected into model group and blank control group through tail vein.Serum follicle stimulating hormone (FSH) and estradiol estradiol E2 (E2) levels were measured on the 14th day after modeling and 2 months after BMSCs transplantation. The location of BMSCs in ovarian tissue and apoptosis of ovarian granulosa cells were observed after transplantation for 2 months, and the number of oocytes obtained after ovulatory hyperstimulation in each group was recorded.Fertilization rate, cleavage rate, blastocyst formation rate, and the number of postcaged pregnancy and offspring with male mice.Results two months after BMSCs transplantation, a large number of green fluorescent cells and labeled BMSCs were found in the interstitial ovary of the transplanted group, but the level of FSH receptor FSH-FSH and the apoptotic index of granulosa cells were significantly lower than those of the model group. The number of oocytes obtained and fertilization rate, cleavage rate and cleavage rate were significantly lower than those in the model group.The blastocyst formation rate in the model group was significantly higher than that in the model group (P 0.05), but the indexes were still significantly lower than that in the blank control group (P 0.05), and there was no pregnancy in the model group, 2 mice in the transplantation group were pregnant, and 42 mice with normal appearance were born in the model group.Conclusion BMSCs can locate in the stroma of ovary, decrease the apoptosis index of granulosa cells, improve the endocrine function of ovary and improve the fertility of mice.
【作者单位】：解放军第105医院生殖医学中心;
【基金】：南京军区医学科技创新课题项目(编号:11Z010)
【分类号】：R711.75

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