外源性阻断RAGE效应对糖尿病小鼠创面中巨噬细胞浸润的影响

发布时间：2018-04-18 05:07

本文选题：糖尿病 + 巨噬细胞　；参考：《上海交通大学学报(医学版)》2017年12期

【摘要】：目的·通过外源性阻断糖基化终末产物受体(RAGE)后,观察RAGE效应对糖尿病小鼠创面愈合过程中巨噬细胞浸润的影响。方法·96只雄性8周龄C57BL/6J小鼠随机分成糖尿病组(n=72)和正常对照组(n=24)。多次小剂量链脲佐菌素腹腔注射诱导糖尿病小鼠模型,并用灭菌9 mm直径环钻于小鼠背部制造全层皮肤缺损创面模型。伤后正常对照组创面局部用0.9%氯化钠溶液(N组);糖尿病组再随机分成3组,糖尿病空白对照组(C组)创面局部外用0.9%氯化钠溶液,糖尿病Ig G对照组(I组)创面局部外用兔Ig G,糖尿病RAGE干预组(R组)创面局部外用RAGE抗体。再于伤后第3日和第7日,给予相同处理。通过大体评估创面愈合情况;于伤后第1、3、7日切取创缘皮肤样本,免疫组织化学染色标记并计数创面中巨噬细胞数量,透射电子显微镜下观察巨噬细胞超微结构。结果·(1)伤后第14日,R组创面愈合面积百分比显著高于C组和I组(均P=0.000)。(2)伤后第1日,N组和R组CD68+巨噬细胞数均显著多于C组(均P=0.000);伤后第14日,N组和R组巨噬细胞数均显著少于C组(均P=0.000)。(3)电子显微镜下对比创面组织的巨噬细胞形态及结构,存在明显不同。结论·糖尿病创面环境中,RAGE效应与创面巨噬细胞浸润数量及形态的异常密切相关,从而参与了糖尿病创面的难愈。
[Abstract]:Objective to investigate the effect of RAGE effect on macrophage infiltration during wound healing in diabetic mice after exogenous blockade of glycosylation end product receptor (rage).Methods 96 male 8-week-old C57BL/6J mice were randomly divided into diabetic group (n = 72) and normal control group (n = 24).The model of diabetic mice was induced by intraperitoneal injection of small dose streptozotocin (STZ), and the full-thickness skin defect wound model was made by sterilizing 9 mm diameter circular drilling on the back of mice.The normal control group was treated with 0.9% sodium chloride solution and the diabetic group was randomly divided into 3 groups: the control group was treated with 0.9% sodium chloride solution, and the control group was treated with 0.9% sodium chloride solution.Diabetic IGG control group (group I) wound local topical use of rabbit IGG, diabetic RAGE intervention group (group R) wound topical application of RAGE antibody.The same treatment is given on the 3rd and 7th day after injury.The wound healing was evaluated roughly, and the wound skin samples were taken on the 3rd and 7th day after injury. The number of macrophages in the wound was labeled with immunohistochemical staining, and the ultrastructure of macrophages was observed under transmission electron microscope.Results 1) the percentage of wound healing area in group R on the 14th day after injury was significantly higher than that in group C and group I (P0. 0000.000). The number of CD68 macrophages in group N and group R was significantly higher than that in group C (P0. 000), and the number of macrophages in group N and group R on the 14th day after injury was significantly higher than that in group C (P 0. 000).The number of macrophages in group C was significantly lower than that in group C (P0. 000, P < 0. 000), and the morphology and structure of macrophages in wound tissue were compared under electron microscope.There are obvious differences.Conclusion the rage effect in diabetic wound environment is closely related to the abnormal number and morphology of macrophage infiltration in the wound surface, thus participating in the refractory wound healing of diabetes mellitus.
【作者单位】：上海交通大学医学院附属第九人民医院急诊科;上海交通大学医学院附属瑞金医院上海烧伤研究所;
【基金】：国家自然科学基金面上项目(81272111,81671917)~~
【分类号】：R587.1

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