乌鲁木齐地区维、汉哮喘患者外周血STAT3、NF-kB基因表达水平差异性研宄

发布时间：2018-04-18 20:43

本文选题：支气管哮喘 + STAT3　；参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:本研究通过检测乌鲁木齐地区维吾尔族、汉族哮喘患者及健康人群外周血中信号传导与转录激活因子3 (STAT3)、核转录因子(NF-kB) mRNA的表达水平及血浆中STAT3、NF-kB的蛋白表达水平,比较基因表达的差异性,以探讨STAT3、NF-kB在哮喘发病中的作用意义。方法:(1)本实验的研究对象取自在新疆医科大学第一附属医院确诊为哮喘急性发作期的患者157例(哮喘组),其中风痰哮51例,热哮52例、冷哮54例。同期选取在新疆医科大学第一附属医院体检的121例健康人群(对照组)。(2)采用实时荧光定量反转录聚合酶链反应(RT-PCR)检测STAT3、NF-kB基因的表达水平。(3)采用酶联免疫吸附法(enzyme-linked immunosorbent,ELISA)检测血清中STAT3、NF-kB的蛋白表达水平。结果:(1)STAT3水平:哮喘组外周血中STAT3水平均高于对照组,具有明显差异(P0.05),其中风痰组、冷哮组、热哮组外周血中STAT3mRNA表达水平也均高于对照组,具有明显差异(P0.05),而风痰组、冷哮组、热哮组三组之间STAT3mRNA表达水平无明显差异(P0.05)。STAT3mRNA表达水平在维吾尔族哮喘患者及汉族哮喘患者有统计学差异(P0.05),而STAT3蛋白表达水平在两组之间无明显差异(P0.05)。(2) NF-kB水平:哮喘组外周血中NF-kB水平高于对照组,具有明显差异(P0.05),其中风痰组、冷哮组、热哮组外周血中NF-kBmRNA表达水平也均高于对照组,具有明显差异(P0.05),而风痰组、冷哮组、热哮组三组之间NF-kBmRNA表达水平无明显差异(P0.05)。NF-kBmRNA及蛋白表达在维吾尔族哮喘患者及汉族哮喘患者之间差异无统计学意义(P0.05)。结论:(1) STAT3及NF-kB参与了哮喘的发病。(2) STAT3可能是引起维吾尔族哮喘与汉族哮喘患病率不同的原因。(3)哮喘急性发作期常见证型风痰哮、冷哮、热哮与STAT3mRNA、NF-kBmRNA关系不明显。
[Abstract]:Objective: to investigate the expression of signal transduction and transcription activator 3 (STAT3) and nuclear transcription factor NF-kB (NF-kB) in peripheral blood of Uygur, Han and healthy subjects in Urumqi, and to detect the protein expression of STAT3NF-kB in plasma.To investigate the role of STAT 3 and NF-kB in the pathogenesis of asthma by comparing the difference of gene expression.Methods the subjects of this study were 157 patients with acute attack of asthma diagnosed in the first affiliated Hospital of Xinjiang Medical University (asthma group, 51 cases of wind-phlegm asthma, 52 cases of heat asthma, 54 cases of cold asthma).At the same time, 121 healthy people (control group, control group) were examined by real-time fluorescence quantitative reverse transcriptase polymerase chain reaction (RT-PCR) to detect the expression level of NF-kB gene in STAT3 and NF-kB gene, and enzyme linked immunosorbent assay (Elisa) was used to detect the expression level of NF-kB gene in 121 healthy people (control group) in the first affiliated Hospital of Xinjiang Medical University.The protein expression of STAT3 and NF-kB in serum was detected by immunosorbent Elisa.Results: the level of STAT3 in peripheral blood of asthma group was higher than that of control group, and there was significant difference (P 0.05). The level of STAT3mRNA in peripheral blood of wind phlegm group, cold asthma group and heat asthma group were also higher than that of control group, and there was significant difference between wind and phlegm group and cold asthma group.There was no significant difference in the expression of STAT3mRNA between the three groups. There was significant difference in the expression of STAT3mRNA between Uygur asthmatic patients and Han asthmatic patients. However, there was no significant difference in the expression of STAT3 protein between the two groups. There was no significant difference between the two groups in the level of NF-kB.The level of NF-kB in peripheral blood was higher than that in control group.The expression of NF-kBmRNA in peripheral blood of wind phlegm group, cold asthma group and heat asthma group were all higher than that of control group, and there was significant difference between wind and phlegm group, cold asthma group, wind phlegm group, cold wheezing group.There was no significant difference in the expression of NF-kBmRNA between the three groups. There was no significant difference between Uygur asthmatic patients and Han asthmatic patients in the expression of P0.05. NF-kB mRNA and protein.Conclusion: STAT3 and NF-kB are involved in the pathogenesis of asthma. STAT3 may be the cause of the different prevalence of asthma in Uygur and Han nationality. (3) there is no significant relationship between STAT3 and STAT3mRNA-NF-kB mRNA in acute stage of asthma with common syndromes of wind-phlegm asthma, cold asthma, heat asthma and STAT3mRNA-NF-kB mRNA.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R562.25

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