代谢综合征患者糖化血红蛋白与HDL亚类分布的相关性研究

发布时间：2018-04-20 15:49

本文选题：代谢综合征 + 糖化血红蛋白　；参考：《南华大学》2015年硕士论文

【摘要】：目的:探讨代谢综合征(MS)患者糖化血红蛋白(Hb A1c)水平与高密度脂蛋白(HDL)亚类分布的关系。方法:收集110例MS患者和108例对照人群血样本,采用高效液相色谱法检测分析受试者Hb A1c的浓度,全自动生化分析仪测定血浆血脂、血糖及载脂蛋白浓度,ELISA试剂盒测定血浆卵磷脂胆固醇酰基转移酶(lecithin cholesterol acyltransferase,LCAT)及胆固醇酯转运蛋白(cholesteryl ester transfer protein,CETP)的浓度,双向电泳-免疫印记检测法测定受试者HDL亚类的相对含量。结果:1.与对照组相比,MS患者Hb A1c、空腹血糖(FPG)、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白B100(apo B100)、preβ1-HDL、HDL3b含量及apo B100/AI和LDL-C/HDL-C比值均显著性增高(P0.01),而高密度脂蛋白胆固醇(HDL-C)、载脂蛋白AI(apo AI)、HDL2b和HDL2a水平显著降低(P0.01),血浆LCAT、CETP含量无显著差异。2.随着Hb A1c水平的升高,MS患者FPG、TC、TG、LDL-C的含量有升高趋势,血浆HDL-C的含量有降低趋势,apo AI含量变化无统计学差异。3.随着Hb A1c水平的升高,MS患者小颗粒的preβ1-HDL含量逐渐升高,大颗粒的HDL2b及HDL2a含量逐渐降低。结论:代谢综合征患者Hb A1c水平升高可加重HDL成熟代谢障碍。
[Abstract]:Objective: to investigate the relationship between HbA1c level and HDL subclass distribution in patients with metabolic syndrome (MS). Methods: blood samples were collected from 110 MS patients and 108 controls. The concentrations of HB A1c were detected by HPLC, and plasma lipids were measured by automatic biochemical analyzer. The concentrations of plasma lecithin cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP) in plasma were determined by Elisa kit. The relative content of HDL subclass was determined by two-dimensional electrophoresis and immunoblotting assay. The result is 1: 1. Compared with the control group, the levels of HB A1c, fasting blood glucose (FPG), triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), apolipoprotein B100 (pre 尾 1-HDLN) HDL3b and the ratio of apo B100/AI to LDL-C/HDL-C in MS patients were significantly higher than those in the control group (P 0.01). The levels of HDL2b and HDL2a in lipoprotein AI(apo were significantly lower than those in control group (P 0.01), but there was no significant difference in CETP content in plasma. With the increase of HbA1c level, the LDL-C content of TGN TGN in MS patients increased, while the plasma HDL-C content decreased. There was no significant difference in ApoAI content. 3. With the increase of HB A1c level, the content of pre 尾 1-HDL in small granules of MS patients increased gradually, while the content of HDL2b and HDL2a in large granules decreased gradually. Conclusion: the increase of HB A 1c level in patients with metabolic syndrome can aggravate the HDL mature metabolic disorder.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R589

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