口服降糖药致低血糖对糖尿病大鼠神经生长因子mRNA表达的影响

发布时间：2018-04-20 23:06

本文选题：低血糖症 + 糖尿病　；参考：《重庆医学》2017年08期

【摘要】：目的观察糖尿病大鼠脑组织神经生长因子(NGF)mRNA表达变化及口服降糖药致低血糖的影响作用。方法64只Wistar大鼠分为对照组(16只)、糖尿病模型组(48只),制备高脂高糖饮食+腹腔注射链脲佐菌素致2型糖尿病大鼠模型,模型组分为非药物组(16只)、口服药物治疗组(16只)和低血糖组(16只),口服药物治疗组和低血糖组大鼠均予格列本脲+二甲双胍灌胃给药,低血糖组剂量为口服药物治疗组5倍,每天1次,连续12d,各组大鼠分别于低血糖反应第3、6、9、12天取脑组织行RT-PCR法NGF mRNA检测。结果与对照组相比,糖尿病模型组3组大鼠脑组织NGF mRNA表达均不同程度下降,低血糖降低最为显著(P0.01),与非药物组比较,口服药物治疗组升高明显(P0.01),低血糖组则进一步降低(P0.01)。结论口服降糖药可有效增强糖尿病大鼠脑组织NGF mRNA表达,而口降糖药致低血糖能使NGF mRNA表达进一步下降,不利于糖尿病神经病变的修复。
[Abstract]:Objective to observe the changes of NGF mRNA expression in brain tissue of diabetic rats and the effect of hypoglycemia induced by oral hypoglycemic drugs. Methods Sixty-four Wistar rats were divided into control group (n = 16) and diabetic model group (n = 48). The model group consisted of 16 rats in non-drug group (n = 16) and 16 rats in oral drug treatment group (n = 16) and hypoglycemia group (n = 16). The rats in oral drug treatment group and hypoglycemia group were given glibenclamide metformin intragastrically, and the dosage in hypoglycemia group was 5 times as that in oral drug treatment group. Once a day for 12 days, the brain tissues of each group were taken from the brain at the 3rd day of hypoglycemia reaction for 12 days. The NGF mRNA was detected by RT-PCR method. Results compared with the control group, the expression of NGF mRNA in the brain tissues of the diabetic model group decreased in varying degrees, and the hypoglycemia decreased most significantly (P 0.01). Compared with the control group, the oral drug treatment group increased significantly (P 0.01), and the hypoglycemia group further decreased the P 0.01 (P 0.01). Conclusion Oral hypoglycemic drugs can effectively enhance the expression of NGF mRNA in the brain of diabetic rats, while hypoglycemia induced by oral hypoglycemia can further decrease the expression of NGF mRNA, which is not conducive to the repair of diabetic neuropathy.
【作者单位】：成都医学院第一附属医院内分泌科;
【基金】：四川省卫生厅科研课题(120478)
【分类号】：R587.2

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