被动吸烟对去势雌鼠骨密度及骨代谢影响的研究
发布时间:2018-04-22 12:23
本文选题:骨质疏松 + 绝经后骨质疏松 ; 参考:《吉林大学》2015年硕士论文
【摘要】:目的: 很长的时间里,人们对于吸烟的危害还主要在于吸烟者自身,而事实上并不如此。因为并不是所有的烟雾都直接进了吸烟者的体内,,很多人认为其中大部分随着呼出的二氧化碳被呼入到周围的环境中被别人吸进去,被动吸烟对人们的健康危害越来越明显尤其是对绝经后女性。被动吸烟可能会加速绝经女性骨代谢,使骨质疏松提前出现,希望通过动物实验研究提供理论依据。 方法: 将40只Wister大白鼠随机分成4组,A组:假手术;B组:去势模型;C组:被动吸烟;D组:去势+被动吸烟。分别于实验1个月、2个月和3个月后测量大鼠代谢生化指标:尿钙及尿肌酐并计算出Ca/Cr比值、血清骨钙素;骨密度测量,行组间对比。 结果: 实验1个月:B组、与A组、C组骨密度、骨钙素及尿Ca/Cr无差异(p0.05);D组与A组、B组、C组骨密度、骨钙素及尿Ca/Cr有差异(p0.05);实验2个月:B组与D组大鼠骨密有差异(p0.05); B组与A、C组骨密度、骨钙素及尿Ca/Cr有差异(p0.05); D组与A、C组在骨密度、骨钙素及尿Ca/Cr有差异(p0.05);实验3个月:B、D之间在骨密度、骨钙素及尿Ca/Cr均有差异(p0.05);同时B组在骨密度、骨钙素及尿Ca/Cr方面显著大于D组; 结论: 1、去势大鼠给予被动吸烟,比单独去势及单独给予被动吸烟更早出现骨代谢及骨密度变化,证实去势大鼠骨密度及骨代谢生化指标对被动吸烟更敏感。被动吸烟行为使去势大鼠比不被动吸烟者更早出现骨质疏松。即绝经后女性的被动吸烟,使其更早出现骨质疏松2、被动吸烟行为与绝经后骨质疏松有时间——效应相关性,实验3个月时,被动吸烟行为的去势大鼠比不吸烟去势大鼠骨质疏松的严重程度,比实验1个月时更严重。提示绝经后被动吸烟时间越长,骨质疏松的程度越严重。
[Abstract]:Objective: For a long time, the danger of smoking was mainly in the smoker himself, but not in fact. Because not all the smoke goes directly into the body of the smoker, many people think that most of it is breathed into the surrounding environment with the exhalation of carbon dioxide being sucked in by others. Passive smoking is more and more harmful to people's health, especially to postmenopausal women. Passive smoking may accelerate bone metabolism in menopausal women and make osteoporosis appear earlier. Methods: Forty Wister rats were randomly divided into 4 groups: sham operation group B: castrated model group C: passive smoking group D: castrated passive smoking group. After 1 month, 2 months and 3 months, the biochemical indexes of rat metabolism were measured: urine calcium and creatinine, the ratio of Ca/Cr, serum osteocalcin, bone mineral density, and the comparison between the two groups. Results: There was no difference in bone mineral density (BMD), osteocalcin and urinary Ca/Cr between group B and group A (1 month), and there was no difference in bone mineral density (BMD) between group D and group A (P 0.05). There was a significant difference in bone density between group B (n = 2) and group D (n = 2), and bone density between group B and group D (n = 2). There were significant differences in bone mineral density (BMD), osteocalcin (BGP) and urinary Ca/Cr (P 0.05) between group D and group A (P 0.05), and there were differences in bone mineral density (BMD), osteocalcin (BGP) and urinary Ca/Cr (P 0.05) between group D and group A (3 months), while in group B (group B), bone mineral density (BMD) was higher than that in group B (P < 0.05). Osteocalcin and urinary Ca/Cr were significantly higher in group D than in group D. Conclusion: 1. The changes of bone metabolism and bone mineral density in ovariectomized rats were earlier than those in ovariectomized rats. It was confirmed that bone mineral density and biochemical indexes of bone metabolism were more sensitive to passive smoking in ovariectomized rats. Passive smoking resulted in osteoporosis in ovariectomized rats earlier than in non-passive smokers. That is, passive smoking in postmenopausal women made them appear osteoporosis 2 earlier. Passive smoking behavior had a time-effect correlation with postmenopausal osteoporosis, and at 3 months of experiment, there was a time-effect correlation between passive smoking behavior and postmenopausal osteoporosis. The severity of osteoporosis in ovariectomized rats with passive smoking behavior was more serious than that in non-smoking castration rats. It suggested that the longer the passive smoking time after menopause, the more serious osteoporosis.
【学位授予单位】:吉林大学
【学位级别】:硕士
【学位授予年份】:2015
【分类号】:R580
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