活动性系统性红斑狼疮患者外周血嗜碱性粒细胞CD62L、BAFF和CD40L表达变化及意义

发布时间：2018-04-25 21:45

本文选题：系统性红斑狼疮 + 嗜碱性粒细胞　；参考：《山东医药》2017年09期

【摘要】：目的探讨活动性系统性红斑狼疮(SLE)患者外周血嗜碱性粒细胞淋巴归巢受体(CD62L)、B淋巴细胞活化刺激因子(BAFF和CD40L)表达变化及意义。方法选取SLE患者57例,根据SLEDAI评分将SLE患者分为活动组(SLEDAI评分≥10分)29例和非活动组(SLEDAI评分10分)28例,选取同期20例查体健康者作为对照组,应用流式细胞术检测SLE患者和正常人外周血CD62L、BAFF和CD40L表达,并采用Spearman法分析其与血清Anti-ds DNA水平的相关性。结果活动组与非活动组外周血CD62L表达水平均高于对照组(P均0.05),且活动组高于非活动组(P0.05)。活动组外周血BAFF和CD40L表达水平均高于对照组和非活动组(P均0.05);而非活动组与对照组BAFF和CD40L表达差异无统计学意义(P均0.05)。SLE患者外周血嗜碱性粒细胞CD62L和CD40L表达与血清Anti-ds DNA抗体水平均呈正相关(r分别为0.34、0.36,P均0.05);而外周血嗜碱性粒细胞BAFF表达与血清Anti-ds DNA抗体水平无关(r=0.26,P=0.14)。结论活动性SLE患者外周血CD62L、BAFF和CD40L表达升高,嗜碱性粒细胞可能通过CD62L介导向次级淋巴器官迁移,通过BAFF和CD40L活化自身反应性B淋巴细胞而产生多种自身抗体,进而参与SLE的发病。
[Abstract]:Objective to investigate the changes and significance of B lymphocyte activation stimulating factor (BAFF and CD40L) expression in peripheral blood basophil lymphoid homing receptor (CD62L) in patients with active systemic lupus erythematosus (SLE). Methods 57 patients with SLE were selected and SLE patients were divided into active group (SLEDAI score > 10) according to SLEDAI score in 29 cases and non active group (SLEDAI evaluation). In 28 cases, 20 healthy subjects were selected as control group at the same time. Flow cytometry was used to detect the expression of CD62L, BAFF and CD40L in peripheral blood of SLE and normal people. The correlation between the level of serum Anti-ds DNA and serum level of serum was analyzed by Spearman. The expression of CD62L in the peripheral blood of the active group and the non active group was higher than that of the control group (P 0.05), The activity group was higher than the non active group (P0.05). The expression level of BAFF and CD40L in the peripheral blood of the active group was higher than that of the control group and the non active group (P 0.05), but there was no significant difference in the expression of BAFF and CD40L in the non active group and the control group (P 0.05). The expression of CD62L and CD40L in peripheral blood basophil and the level of Anti-ds DNA antibody in the peripheral blood of the patients with.SLE were all present. The positive correlation (r was 0.34,0.36, P 0.05 respectively), but the expression of BAFF in peripheral blood basophil was not related to the level of serum Anti-ds DNA antibody (r=0.26, P=0.14). Conclusion the expression of CD62L, BAFF and CD40L in peripheral blood of active SLE patients increased. Reactive B lymphocytes produce multiple autoantibodies and participate in the pathogenesis of SLE.

【作者单位】：广东医科大学附属医院;
【基金】：国家自然科学基金资助项目(81202346) 广东省自然科学基金项目(S2012040006216) 广东省医学科学技术研究基金项目(A2015520)
【分类号】：R593.241

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