高迁移率族蛋白B1和Toll样受体4与哮喘气道炎症的关系及维生素D的作用

发布时间：2018-04-26 02:06

本文选题：高迁移率族蛋白B(HMGB) + Toll样受体(TLR)　；参考：《西安交通大学学报(医学版)》2017年04期

【摘要】：目的探讨高迁移率族蛋白B1(HMGB1)和Toll样受体4(TLR4)与气道炎症的关系及维生素D的作用。方法 BALB/c小鼠随机分为对照组、哮喘组和1,25-(OH)2D3干预组(每组8只)。采用HE染色观察小鼠肺组织病理学变化并应用计算机图像系统测定支气管壁厚度,采用免疫组化法观察肺组织HMGB1和TLR4表达,同时收集支气管肺泡灌洗液(BALF)和外周血,将BALF进行细胞学检测,用酶联免疫吸附法(ELISA)测定BALF和外周血中HMGB1、TLR-4、白介素-4(IL-4)和干扰素-γ(IFN-γ)含量。结果哮喘组小鼠肺组织HMGB1和TLR4表达较强,干预组表达较弱。哮喘组BALF中白细胞总数及嗜酸性粒细胞、中性粒细胞和淋巴细胞比例明显升高,单核/巨噬细胞比例明显下降(均为P0.05);干预组BALF中白细胞总数及嗜酸性粒细胞、中性粒细胞和淋巴细胞比例明显下降,单核/巨噬细胞比例明显上升(均为P0.05)。哮喘组BALF和外周血中HMGB1、TLR4和IL-4含量均明显高于对照组,IFN-γ含量均明显低于对照组(均为P0.05);干预组BALF和外周血中HMGB1、TLR4和IL-4含量均明显低于哮喘组,IFN-γ含量明显高于哮喘组(均为P0.05)。BALF中HMGB1和TLR4含量分别与细胞总数和IL-4浓度均呈正相关(r1为0.796、0.730;r2为0.695、0.648;均为P0.05)。结论 HMGB1和TLR4与气道炎症和免疫紊乱有关;适量1,25-(OH)2D3可减轻气道炎症,可能与调节Th1/Th2细胞平衡有关。
[Abstract]:Objective to investigate the relationship between high mobility group protein B1 (HMGB1) and Toll like receptor 4 (TLR4) and airway inflammation and the role of vitamin D. Methods BALB/c mice were randomly divided into three groups: control group, asthma group and OH2D3 intervention group (8 rats in each group). The histopathological changes of lung tissue were observed by HE staining, the thickness of bronchial wall was measured by computer image system, the expressions of HMGB1 and TLR4 in lung tissue were observed by immunohistochemical method, and the bronchoalveolar lavage fluid (BALF) and peripheral blood were collected. The levels of HMGB1TLR-4, IL-4IL-4 and IFN- 纬) in BALF and peripheral blood were determined by enzyme-linked immunosorbent assay (Elisa). Results the expression of HMGB1 and TLR4 was stronger in asthma group than that in intervention group. In asthma group, the percentage of leukocytes and eosinophils, neutrophils and lymphocytes in BALF was significantly increased, and the ratio of monocyte / macrophage was significantly decreased (P 0.05), the total number of leukocytes and eosinophilic granulocytes in BALF in intervention group, and the percentage of eosinophils in BALF in intervention group were significantly increased. The percentage of neutrophils and lymphocytes decreased significantly, and the ratio of mononuclear / macrophage increased significantly (both P0.05A). The levels of HMGB1TLR4 and IL-4 in asthma group were significantly higher than those in control group (P 0.05), and the levels of BALF and HMGB1TLR4 and IL-4 in peripheral blood in intervention group were significantly lower than those in asthma group (P < 0.05). The content of HMGB1TLR4 and IL-4 in peripheral blood of asthma group was significantly higher than that of asthma group (P < 0.05). The contents of HMGB1 and TLR4 in P0.05).BALF were positively correlated with the total number of cells and the concentration of IL-4, respectively. Conclusion HMGB1 and TLR4 may be associated with airway inflammation and immune disorder, and moderate dose of 1 ~ (25 ~ (-1)) OH ~ (2 +) D _ (3) can alleviate airway inflammation, which may be related to regulating the balance of Th1/Th2 cells.
【作者单位】：郑州大学第三附属医院儿科;
【基金】：河南省科技厅2014年度科技攻关项目(No.142102310542)~~
【分类号】：R562.25

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