鞣花酸预防高脂饮食诱导的大鼠肥胖的作用研究

发布时间：2018-04-26 07:47

本文选题：鞣花酸 + 肥胖　；参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:通过鞣花酸干预单一的高脂饮食诱导的大鼠肥胖,探讨其对肥胖的影响及作用。方法:健康SPF级雄性SD大鼠56只按体重随机分为七组,对照组,模型组,鞣花酸高剂量组(30mg/kg),鞣花酸低剂量组(10mg/kg),盐酸二甲双胍缓释片阳性对照药(89.25 mg/kg),奥利司他阳性对照药(37.8 mg/kg)和硫普罗宁肠溶片阳性对照药(47.25 mg/kg),每周测量一次体重,两次进食量,分别在第12周和第25周量取大鼠体长。第25周时,分别腹腔注射葡萄糖溶液和胰岛素溶液,检测血糖水平;用超声诊断仪测量肾横切面中部前方脂肪层厚度;处理动物取血检测HDL-C、LDL-C、TC和TG水平;取附睾周和肾周白色脂肪组织和肩胛处棕色脂肪组织进行HE染色观察脂肪细胞形态,并计算白色脂肪系数;取肝脏计算肝脏系数后HE染色进行细胞形态观察;Western blot法检测附睾周白色脂肪组织中PPAR γ、C/EBP-β、C/EBP-α、UCP-1和PGC-1α蛋白的表达水平。结果:1.鞣花酸高剂量组(417.06±42.93),鞣花酸低剂量组(395.61±30.83),盐酸二甲双胍缓释片组(412.07±49.26),奥利司他组(408.09±52.18)和硫普罗宁肠溶片组(395.93±63.43)较模型组(559.45±59.46)大鼠体重均明显降低(p0.05),但组间进食量无差异(p0.05);2.腹腔注射葡萄糖溶液后,与模型组(158.63 ±6.00,13982.63±933.17)比较,鞣花酸高(127.13 ±27.82,12423.38 ± 1299.97)、低剂量组(121.63 ±7.84,12412.29 ± 872.91)30 min时的血糖水平以及曲线下面积均显著降低(p0.05);腹腔注射胰岛素溶液后,鞣花酸高(2.59 ±0.16,439.50 ±22.65)、低剂量组(3.21 ±0.22,457.81 ±27.02)30 min时的血糖水平以及曲线下面积较模型组(4.65 ±0.35,503.81 ±26.26)亦均显著降低(p0.05);3.鞣花酸高(3.97±0.42)、低剂量组(3.51±0.38)HDL-C 水平较模型组(2.9±0.59)均显著升高(p0.05);4.与模型组(0.22±0.03)比较,鞣花酸高(0.05±0.01)、低剂量组(0.06±0.03)肾周脂肪层厚度明显减少(p0.05);高(1.96±0.16)、低剂量组(2.08±0.13)的白色脂肪系数较模型组(4.26±0.19)也明显降低(p0.05)。HE结果显示,与模型组比较,鞣花酸干预后,附睾周白色脂肪细胞体积明显变小且数量显著减少,肩胛处棕色脂肪细胞体积变小且组织内毛细血管量增加,肝脏内空泡明显减少,肝脏细胞排列趋于规则。5.鞣花酸高剂量组大鼠附睾周脂肪组织中PPAR γ(0.22±0.01)、C/EBP-β(0.25±0.09)和 C/EBP-α(0.20±0.06)蛋白表达量较模型组(0.44±0.06,0.45±0.04,0.50±0.09)均显著降低(p0.05);而 UCP-1(0.38±0.05)和 PGC-1α(0.43±0.08)蛋白表达量较模型组(0.18±0.04,0.16±0.06)则显著增加(p0.05)。结论:鞣花酸高、低剂量均具有良好的降糖作用,可以改善胰岛素抵抗,减少体内白色脂肪的积累,鞣花酸高剂量降低脂质合成关键蛋白表达的同时增加棕色脂肪标志蛋白的表达,可能具有促使体内白色脂肪组织棕色样变的作用,和预防高脂饮食诱导的肥胖的形成。
[Abstract]:Aim: to investigate the effect of tannic acid on obesity induced by high fat diet in rats. Methods: Fifty-six male SD rats of SPF grade were randomly divided into seven groups according to their body weight: control group, model group, and control group. The high dose of tannic acid was 30 mg / kg / kg, the low dose group of tannin was 10 mg / kg / kg, the metformin hydrochloride sustained release tablet was 89.25 mg / kg / kg, the orlistat positive control drug was 37.8 mg / kg) and the tiopronin enteric-coated drug was 47.25 mg / kg 路kg ~ (-1). The body length of rats was taken at the 12th week and the 25th week, respectively. At the 25th week, glucose solution and insulin solution were injected intraperitoneally to detect the blood glucose level, the thickness of the anterior adipose layer in the middle of the cross section of kidney was measured by ultrasonic diagnostic instrument, and the HDL-CU LDL-CU TC and TG levels were measured by blood collection from the treated animals. The white adipose tissue around epididymis and kidney and brown adipose tissue of scapula were stained with HE to observe the morphology of adipocytes and calculate the coefficient of white fat. The expression levels of PPAR 纬 C / EBP- 尾 C / EBP- 伪 UCP-1 and PGC-1 伪 in white adipose tissue around epididymis were detected by using HE staining after liver coefficient was calculated. The result is 1: 1. The rats in the high dose group of tannic acid 417.06 卤42.93%, the low dose group of tannic acid 395.61 卤30.83L, the metformin hydrochloride sustained-release tablet group 412.07 卤49.26, the olesta group 408.09 卤52.18) and the tiopronin enteric-coated tablet group 395.93 卤63.43) the body weight of the rats decreased significantly compared with the model group (559.45 卤59.46), but there was no difference in food intake between the two groups. After intraperitoneal injection of glucose solution, compared with model group (158.63 卤6.00 卤13982.63 卤933.17), the level of blood glucose and the area under the curve decreased significantly at 121.63 卤7.8412.29 卤872.9130 min in the low dose group and 127.13 卤27.82 卤12423.38 卤1299.97 in the low dose group, respectively, and the area under the curve decreased significantly after intraperitoneal injection of insulin solution. The blood glucose level and area under the curve of the low dose group (3.21 卤0.22457.81 卤27.02U 30 min) were significantly lower than that of the model group (4.65 卤0.35503.81 卤26.26). The blood glucose level of the low dose group was also significantly lower than that of the model group (4.65 卤0.35503.81 卤26.26). The level of 0.38)HDL-C in low dose group (3.51 卤0.59) was significantly higher than that in model group (3. 97 卤0. 42) (P < 0. 05). Compared with the model group (0.22 卤0.03), the thickness of perirenal adipose layer decreased significantly in the tannic acid group (0.05 卤0.01g) and the low-dose group (0.06 卤0.03), and the white fat coefficient of the high-dose group (1.96 卤0.16) was significantly lower than that of the model group (4.26 卤0.19). The results showed that compared with the model group, the white fat coefficient of the model group was significantly lower than that of the model group. The volume of white adipocytes around epididymis became smaller and the number of white adipocytes decreased significantly, the volume of brown adipocytes in scapula became smaller and the amount of capillaries in tissues increased, the vacuoles in liver decreased obviously, and the arrangement of liver cells tended to be regular. 5. Compared with model group, the expression levels of PPAR 纬 -0.22 卤0.01C / EBP- 尾 0.25 卤0.09) and C / EBP- 伪 0.20 卤0.06) in the high dose group were significantly lower than those in the model group (0.44 卤0.06 卤0.04 卤0.04 卤0.50 卤0.09), while the expression of UCP-1(0.38 卤0.05) and PGC-1 伪 (0.43 卤0.08) were significantly higher than those in the model group (0.18 卤0.04 卤0.16 卤0.06). Conclusion: both high and low doses of tannic acid have good hypoglycemic effect, which can improve insulin resistance and reduce the accumulation of white fat in body. The high dose of tannic acid can decrease the expression of key protein of lipid synthesis and increase the expression of brown fat marker protein, which may promote the brown appearance of white adipose tissue in vivo and prevent the formation of obesity induced by high fat diet.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R589.2

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