Nesfatin-1对体外培养卵泡黄素化颗粒细胞分泌雌、孕激素的影响

发布时间：2018-04-27 03:14

  本文选题：多囊卵巢综合征 + Nesfatin-1　； 参考：《滨州医学院》2015年硕士论文

【摘要】：目的：探讨不同浓度Nesfatin-1对体外培养人卵泡黄素化颗粒细胞分泌雌激素(E2)、孕激素(P)的影响。方法：筛选20例在滨州医学院附属医院生殖医学科接受体外受精-胚胎移植(IVF-ET)的不孕患者,分成PCOS组20组和对照组15组,采用超促排卵方案,取卵日收集成熟卵母细胞周围的卵丘颗粒细胞,加入含血清培养液(DMEM+10%胎牛血清)体外培养卵泡黄素化颗粒细胞48h后传代,颗粒细胞计数并调整细胞浓度,按2X10S/ml密度接种于24孔培养板,加入不同浓度(1X10-7M、1X10-9 M、1X10-11 M、0)Nesfatin-1的无血清培养液(DMEM)培养24h,收集上清液至-80℃冰箱冻存。采用化学发光法测定颗粒细胞培养液中E2、P的含量。结果：1、体外培养黄素化颗粒细胞加入不同浓度的(1X10-7M、1X10-9M、1X10-11M、0)Nesfatin-1共培养24h后,我们发现：对照组黄素化颗粒细胞生成E2水平随Nesfatin-1浓度增加有增加趋势,各组间有统计学差异。同样PCOS组黄素化颗粒细胞生成E2水平随Nesfatin-1浓度增加而增加。且在不同Nesfatin-1浓度中,PCOS组黄素化颗粒细胞E2生成水平均明显低于对照组,差异具有统计学意义(P0.05)。2、体外培养黄素化颗粒细胞加入不同浓度的(1X10-7M、1X10-9M、1X10-11M、0)Nesfatin-1共培养24h后,我们发现：PCOS组黄素化颗粒细胞生成P水平加入Nesfatin-1后与基础状态比较明显增加,差异有统计学意义(P0.05),对照组黄素化颗粒细胞生成P水平随Nesfatin-1浓度增加与基础状态比较差异均无有统计学意义(P0.05)。在不同Nesfatin-1浓度中,对照组黄素化颗粒细胞生成P水平均明显高于PCOS组,差异具有统计学意义(P0.05)。结论：Nesfatin-1能促进人卵泡黄素化颗粒细胞分泌雌二醇,且促进作用随Nesfatin-1浓度的增加有增加趋势；对PCOS组孕酮的分泌有促进作用,对正常组P分泌无明显影响,这可能与颗粒细胞所处时期及是否有FSH存在有关。
[Abstract]:Aim: to investigate the effects of different concentrations of Nesfatin-1 on the secretion of estradiol E2 and progesterone by human follicular luteinized granulosa cells in vitro. Methods: twenty infertile patients who received IVF-ETT in the Department of Reproductive Medicine, affiliated Hospital of Binzhou Medical College were selected and divided into two groups: PCOS group (n = 20) and control group (n = 15). The cumulus granulosa cells around the mature oocytes were collected on the day of oocyte extraction. The follicular luteinized granulosa cells were cultured in vitro with DMEM 10% fetal bovine serum. The granulosa cells were counted and the cell concentration was adjusted. According to the density of 2X10S/ml, we inoculated on 24-well culture plate and added different concentrations of 1X10-7MnlX10-9 M10-9 MX10-11 M10-11 Nesfatin-1 serum-free medium for 24 hours. The supernatant was collected and stored in refrigerator at -80 鈩，

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