合并2型糖尿病乳腺癌患者肿瘤组织中FOXC2的表达情况及其临床意义

发布时间：2018-04-29 08:38

  本文选题：乳腺癌 + 型糖尿病　； 参考：《东南大学学报(医学版)》2017年04期

【摘要】：目的:探讨FOXC2在合并2型糖尿病乳腺癌患者肿瘤组织中的表达情况,并分析其临床意义。方法:选取2013年1月至2015年12月77例合并2型糖尿病乳腺癌患者为实验组,另取80例非2型糖尿病乳腺癌患者作为对照组。手术切取患者肿瘤组织,对一部分标本提取组织RNA,利用反转录-聚合酶链反应(reverse transcription PCR,RT-PCR)技术检测两组患者癌组织中FOXC2 mRNA表达水平;对另一部分标本石蜡切片行ER、PR、Her-2、FOXC2免疫组化检测,对比免疫组化FOXC2表达结果与RT-PCR结果的一致性。比较两组乳腺癌分子分型构成比的差异,并做FOXC2表达与三阴乳腺癌(基底细胞样型)的相关性分析。通过Kaplan Meier-plotter法分析FOXC2与乳腺癌患者预后的相关性。结果:RT-PCR结果显示,实验组癌组织FOXC2 mRNA表达水平显著高于对照组(P0.05)。免疫组化的结果显示,FOXC2的表达水平与RT-PCR结果一致。实验组luminal A型占比为22.1%(17/77),luminal B型为18.2%(14/77),Her-2过表达型为24.7%(19/77),基底细胞样型为35.1%(27/77);对照组luminal A型占比为37.5%(30/80),luminal B型为30.0%(24/80),Her-2过表达型为16.3%(13/80),基底细胞样型为16.3%(13/80)。两组患者luminal A型、luminal B型及基底细胞样型占比差异有统计学意义(P0.05)。两组患者FOXC2 mRNA表达水平与患者基底细胞样型表型呈显著正相关性(P0.05)。Kaplan Meier-plotter结果显示,高表达FOXC2乳腺癌患者预后差。结论:FOXC2是2型糖尿病致恶性肿瘤的潜在关键因子。
[Abstract]:Objective: to investigate the expression of FOXC2 in breast cancer with type 2 diabetes mellitus and its clinical significance. Methods: from January 2013 to December 2015, 77 cases of breast cancer with type 2 diabetes were selected as experimental group and 80 cases of non-type 2 diabetic breast cancer as control group. Tissue RNAs were extracted from some specimens, and the expression of FOXC2 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) technique, and the expression of FOXC2 mRNA was detected in the other paraffin sections. The results of immunohistochemical FOXC2 expression were consistent with those of RT-PCR. The difference of molecular type composition ratio between the two groups was compared, and the correlation between the expression of FOXC2 and the basal cell-like type of trinegative breast cancer was analyzed. The correlation between FOXC2 and prognosis of breast cancer patients was analyzed by Kaplan Meier-plotter method. Results the expression of FOXC2 mRNA in cancer tissues of the experimental group was significantly higher than that of the control group (P 0.05). Immunohistochemical results showed that the expression level of FOXC2 was consistent with that of RT-PCR. In the experimental group, the proportion of luminal A was 22.1g / 77luminal B was 18.2and the over-expression of Her-2 was 24.775 / 77, and that of basal cell was 35.127 / 77; in the control group, the proportion of luminal A was 37.5 / 80luminal B, the over-expression of Her-2 was 16.3 / 80, and the base-like type was 16.313 / 80 / 80. There were significant differences in luminal A type luminal B and basal cell-like type between the two groups (P 0.05). There was a significant positive correlation between the expression of FOXC2 mRNA and basal cell-like phenotype in the two groups. The results of P0.05. Kaplan Meier-plotter showed that the prognosis of the patients with high expression of FOXC2 was poor. Conclusion:% FOXC2 is a potential key factor in malignant tumor caused by type 2 diabetes mellitus.
【作者单位】： 海南医学院附属医院内分泌科;
【分类号】：R587.1;R737.9
，

本文编号：1819200