类风湿性关节炎新型自身抗原视黄醇结合蛋白1类似蛋白相关分析研究

发布时间：2018-04-30 10:57

本文选题：类风湿性关节炎 + 自身抗原　；参考：《中国免疫学杂志》2017年07期

【摘要】：目的:类风湿性关节炎(RA)是一种全身性自身免疫性疾病,但其发病原因尚不清楚。因此,迫切需要找到诱发此病的相关抗原,但国内外鲜有关于新的自身抗原的报道,本课题组运用克隆技术发现新型自身抗原。方法:从滑膜细胞中提取mRNA,构建c DNA文库,取类风湿性关节炎患者关节滑膜液中的抗体作为探针,筛选c DNA基因库,得到阳性克隆后测序分析,寻找已知相似基因,进而分析蛋白质的构造和Northern印迹杂交(Northern blotting)。结果:发现新型自身抗原c DNA克隆,其与视黄醇结合蛋白1(RBP1)有36.5%的氨基酸序列相同,并发现AT-rich interaction domain,chromo domain,A+T-hook以及TAF homology domain这些特殊的蛋白质构造。因此将其命名为视黄醇结合蛋白1类似蛋白(RBP1 like protein,Rbik)。Rbik RNA不仅表达于滑膜,也表达于心脏、小肠、肌肉等多个器官组织中。结论:此自身抗原的发现可为类风湿性关节炎的实验诊断和治疗提供新的可能指标和靶点。
[Abstract]:Objective: rheumatoid arthritis (RA) is a systemic autoimmune disease. Therefore, there is an urgent need to find the antigens associated with the disease, but there are few reports about new autoantigens at home and abroad. Our team uses cloning technology to find new autoantigens. Methods: mRNAs were extracted from synovial cells and c DNA library was constructed. The antibody in synovial fluid of rheumatoid arthritis patients was used as a probe to screen c DNA gene bank. Then the protein structure and Northern blotting hybridization were analyzed. Results: a novel autoantigen, c DNA clone, was found to have the same amino acid sequence as retinol binding protein 1 (RBP1), and the special protein structure of AT-rich interaction domainmochromo domaina T-hook and TAF homology domain were also found. Therefore, it was named retinol binding protein-1 similar protein RBP1 like RNA expressed not only in synovium, but also in heart, small intestine, muscle and other organs. Conclusion: the discovery of this autoantigen may provide new markers and targets for the experimental diagnosis and treatment of rheumatoid arthritis.
【作者单位】：西藏民族大学;西藏民族大学生命科学基础实验室;
【基金】：国家自然科学基金资助项目(81560732,81560668) 西藏自治区自然科学基金项目(2015ZR-13-18) 西藏自治区高校青年教师创新支持计划项目(QC2015-61)
【分类号】：R593.22

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