TLR4基因敲除对小鼠内脏脂肪组织免疫细胞及脂肪因子的影响

发布时间：2018-05-11 22:18

本文选题：Toll样受体 + 脂肪因子　；参考：《中国免疫学杂志》2017年05期

【摘要】：目的:探讨TLR4基因敲除对小鼠免疫细胞及脂肪因子的影响。方法:取20周龄的雄性野生型C57BL/6小鼠和TLR4~(-/-)小鼠的脾脏和附睾脂肪组织,分离细胞,用流式检测F4/80、CD11b、CD11c、CD3、CD4、CD8分子的表达;q PCR检测附睾脂肪组织内IL-6、HMGB1、TNF-α、脂联素和抵抗素的表达。结果:与野生型C57BL/6小鼠相比,TLR4~(-/-)小鼠脾脏和附睾脂肪组织中M1型(F4/80~+CD11b~+CD11c~+)巨噬细胞比例上升(P0.05),M2型(F4/80~+CD11b~+CD11c~-)巨噬细胞比例下降(P0.05),这种趋势在附睾脂肪组织中表现更为显著。同时发现附睾脂肪组织中CD4~+T细胞比例下降(P0.05),CD8+T细胞比例上升(P0.05);IL-6、HMGB1、抵抗素表达升高(P0.05);TNF-α和脂联素表达降低(P0.05)。结论:TLR4基因敲除可导致内脏脂肪组织脂肪因子和免疫细胞的紊乱。
[Abstract]:Objective: to investigate the effect of TLR4 knockout on immune cells and fat factors in mice. Methods: the spleen and epididymal adipose tissues were isolated from male wild-type C57BL/6 mice and TLR4- / -r-mice at the age of 20 weeks. The expressions of IL-6HMGB1TNF- 伪, adiponectin and resistin in epididymal adipose tissues were detected by flow cytometry. Results: compared with wild-type C57BL/6 mice, the ratio of M _ 1 type F _ 4 / F _ 4 ~ CD _ 11b ~ CD _ 11c ~) macrophages in the spleen and epididymal adipose tissue of TLR4 / r ~ (-) mice was increased, and the percentage of M _ (1) F _ (4 / 80) ~ CD _ (11) C ~ (-) M _ (2) M _ (2) F _ (4 / 80 ~ CD _ (11) b ~ CD _ (11) C ~ (-) macrophages decreased significantly in epididymal adipose tissue, which was more significant in epididymal adipose tissue. At the same time, it was found that the percentage of CD4- T cells in adipose tissue of epididymis was decreased. The percentage of CD8 T cells in epididymal adipose tissue was increased, the expression of resistin was increased, the expression of adiponectin was decreased, and the expression of adiponectin was decreased. Conclusion the knockout of the TLR4 gene may lead to the disturbance of adipogenic factors and immune cells in visceral adipose tissue.
【作者单位】：新乡医学院基础医学院/河南省分子诊断与医学检验技术协同创新中心;
【基金】：2015年地方高校国家级大学生创新创业训练计划项目(201510472002) 河南省自然科学基金(162300410225)(14A310016) 河南省教育厅科学技术研究重点项目(14A310016)
【分类号】：R589.2

【相似文献】