HIV新发感染者1型抗体蛋白印迹带型分析

发布时间：2018-05-14 22:39

本文选题：HIV-型抗体 + 蛋白免疫印迹法　；参考：《现代预防医学》2017年17期

【摘要】：目的分析HIV-1抗体阳性新发感染者和长期感染者蛋白印迹带型变化,探讨它们的带型分布特征。方法对2014-2016年湛江市HIV-1抗体阳性的男男人群进行新发感染检测,确定新发感染组和长期感染组,对两组的蛋白印迹带型进行统计分析。结果膜蛋白gp41、gp120、gp160、酶蛋白p66和核心蛋白p24阳性率最高(总阳性率分别为94.7%、100.0%、100.0%、96.0%、100.0%),其次是酶蛋白p31、p51(总阳性率分别为84.0%、85.3%),核心蛋白p17(52.0%)和前体蛋白p55(24.0%)最低;新发感染者各带型阳性率总体上比长期感染者低,具体是膜蛋白gp120、gp160和核心蛋白p24始终保持100.0%阳性率,酶蛋白p66稍有下降(100.0%降到94.7%),而其它带型阳性率均有不同程度升高,且各带型在新发感染和长期感染中差异没有统计学意义(P0.05);新发感染者缺失p17+p55(61.1%)比长期感染者(36.8%)要高很多(P=0.070)。结论带型p17+p55的缺失可能提示为新发感染者(P=0.070),待继续收集资料进一步阐明。
[Abstract]:Objective to analyze the changes of Western blotting patterns in new and long term infection with HIV-1 antibody positive, and to explore their distribution characteristics. Methods the new infection was detected in the male group with positive HIV-1 antibody in Zhanjiang city from 2014 to 2016. The new infection group and the long-term infection group were determined. The Western blotting patterns of the two groups were statistically analyzed. Results the positive rates of membrane protein gp41 / gp120 / gp160, enzyme protein p66 and core protein p24 were the highest (the total positive rates were 94.770 / 100.010 / 100.0and 96.00 / 100.050, respectively), followed by p31p51.The total positive rates were 84.085.3s (the total positive rates were 84.085.3a, and the core protein p1752.0) and the precursor protein p5524.0 were the lowest. The positive rate of each band type of newly infected patients was lower than that of long-term infection. The positive rate of membrane protein gp120 gp160 and core protein p24 was 100.0%, while the positive rate of enzyme protein p66 decreased slightly from 100.0% to 94.7%, while the positive rate of other bands increased in varying degrees. There was no significant difference between each band type in new infection and long-term infection (P 0.05), but the loss of p17 p55 (61.1) in newly infected patients was much higher than that in long-term infection (36.8%). Conclusion the deletion of banded p17 p55 may indicate that the newly infected patients may be infected with P0.070, and further data will be collected for further elucidation.
【作者单位】：广东医科大学附属医院感染内科;广东省湛江市疾病预防控制中心检验科;
【分类号】：R512.91

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