肉桂醛促进高糖条件下皮肤成纤维细胞迁移及机制研究

发布时间：2018-05-16 12:54

本文选题：肉桂醛 + 高糖　；参考：《第三军医大学学报》2017年05期

【摘要】：目的探讨肉桂醛(cinnamic aldehyde,CA),核因子E2相关因子(nuclear factor erythroid2-related factor,NRF2)通路激动剂对高糖条件下成纤维细胞迁移的影响及可能机制。方法体外培养HS27细胞,分为正常对照组(NG,5.5 mmol/L)、高糖组(HG,30 mmol/L)、高糖(30 mmol/L)+肉桂醛(20μmol/L)组、高糖(30 mmol/L)+Con siRNA组、高糖(30 mmol/L)+NRF2 siRNA组。迁移实验观察CA对高糖条件下HS27迁移的影响。采用western blot分析CA干预后NRF2及下游HO-1,增殖和凋亡相关蛋白P21和P53的表达情况。2',7'-二氯荧光素二醋酸(DCF)法检测CA对高糖条件下细胞内活性氧簇(reactive oxygen species,ROS)水平的影响。采用NRF2siRNA抑制NRF2通路,观察HS27细胞迁移和氧化应激的变化。结果与正常对照组相比,HS27在高糖情况下,迁移能力明显降低(P0.05),CA干预后,高糖对HS27迁移能力的抑制作用得到改善(P0.05)。进一步研究发现,CA上调NRF2和HO-1,减轻高糖导致的ROS增加([(4 360 935±1 445 723)vs(1 982 673±208 109),P0.05])。然而,高糖条件下敲除NRF2后,细胞内ROS产生进一步增多,并加剧对成纤维细胞迁移能力的抑制[(0.046±0.018)vs(0.318±0.032),P0.05]。结论 CA促进高糖情况下成纤维细胞迁移、缓解氧化应激损伤,并有可能部分依赖于NRF2通路激活。
[Abstract]:Objective to investigate the effect and possible mechanism of cinnamic factor erythroid2-related factor NRF2 pathway agonist on fibroblast migration under high glucose condition. Methods HS27 cells were cultured in vitro and divided into normal control group (5. 5 mmol / L), high glucose group (30 mmol / L) cinnamaldehyde (20 渭 mol / L), high glucose group (30 mmol / L) Con siRNA, and high glucose group (30 mmol / L) NRF2 siRNA. The effect of CA on HS27 migration under high glucose was observed. The expression of proliferative and apoptosis-related proteins P21 and p53 after CA intervention and its downstream HO-1 were analyzed by western blot. The effect of CA on the level of reactive oxygen speciesrose in cells under high glucose condition was detected by the method of DFC. NRF2siRNA was used to inhibit NRF2 pathway to observe the migration of HS27 cells and the changes of oxidative stress. Results compared with the control group, the migration ability of HS27 was significantly decreased after the intervention of P0.05 and CA, and the inhibitory effect of high glucose on the migration ability of HS27 was improved. Further study showed that CA upregulated NRF2 and HO-1, and alleviated the increase of ROS induced by high glucose ([4360935 卤1 445 723)vs(1 982,673 卤208.109] P 0.05). However, after knockout of NRF2 in high glucose condition, intracellular ROS production was further increased, and the inhibition of fibroblast migration was increased [0.046 卤0.018)vs(0.318 卤0.032]. Conclusion CA can promote the migration of fibroblasts and alleviate oxidative stress injury under high glucose, and it may be partly dependent on the activation of NRF2 pathway.
【作者单位】：第三军医大学新桥医院内分泌科;
【基金】：国家自然科学基金面上项目(81471039);国家自然科学基金青年科学基金项目(81500647) 重庆市集成示范计划项目(2013jcsfc10001-5)~~
【分类号】：R285

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