HIV-1抗体阳性者WB带型及其在不同临床分期中的特征分析

发布时间：2018-05-18 13:02

本文选题：HIV-抗体 + WB带型　；参考：《复旦学报(医学版)》2017年05期

【摘要】：目的分析HIV-1抗体阳性者血清中病毒蛋白的变化,探讨不同临床分期HIV-1抗体阳性者Western blot(WB)带型特点。方法应用WB检测HIV-1感染者体内特异性抗体,流式细胞仪检测CD4+T淋巴细胞计数。结果 208例HIV-1抗体阳性者中,男性193例(92.79%),同性性接触感染163例(78.37%)。WB特异性条带gp160、gp120、gp41、p66、p51、p31、p24维持较高的抗体阳性率,在不同临床分期间差异无统计学意义;异性性行为感染者gag基因编码蛋白的抗体p55条带的出现率高于同性性行为感染者(P0.05);全带型(1)、次全带型(2)(缺失p55)、次全带型(3)(缺失p39)、次全带型(4)(缺失p55和p39)、次全带型(5)(缺失p55、p39和p17)5种带型为常见带型,全带型出现率最高(44.2%)。结论 HIV-1抗体阳性者的CD4+T淋巴细胞计数有助于判断机体免疫状态、确定疾病分期及监测疾病进展。闵行区艾滋病的防治重点为外来流动人员及男男性行为人群。
[Abstract]:Objective to analyze the changes of viral protein in serum of HIV-1 antibody positive patients, and to explore the characteristics of Western blotWB bands in patients with HIV-1 antibody positive in different clinical stages. Methods WB was used to detect specific antibodies in HIV-1 infected patients and CD4 T lymphocytes were detected by flow cytometry. Results among the 208 cases with positive HIV-1 antibody, 193 cases were male and 163 cases had same sex contact infection. The specific band gp160 / gp120 / gp41 / p66p51 / p31 / p24 maintained a high positive rate of antibody, and there was no significant difference between the two groups in different clinical stages. The prevalence of p55 bands of gag gene coding protein in heterosexual infections was higher than that in same-sex patients (P0.05A), while the prevalence of P55 bands was higher in heterosexual infections than that in same-sex patients, and the prevalence of P55 (deletion of p55, p39) was higher than that of P0.05in patients with heterosexual sex, while the prevalence of P55 and p39 in heterosexual individuals was higher than that of P0.05 in patients with heterosexual behavior. There are 5 banding patterns in the absence of p55, p39 and p17, which are common banding patterns. The highest occurrence rate of the whole belt type was 44.2%. Conclusion the count of CD4 T lymphocytes in patients with positive HIV-1 antibody is helpful to judge the immune status, determine the stage of the disease and monitor the progress of the disease. Minhang District AIDS prevention and control focus for migrant migrants and men who have sex with men.
【作者单位】：上海市闵行区疾病预防控制中心;复旦大学生命科学学院遗传学研究所;复旦大学公共卫生学院流行病学教研室;
【基金】：上海市闵行区自然科学研究课题(2015MHZ022) 上海市研究生教育创新计划~~
【分类号】：R512.91

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