骨硬化蛋白单克隆抗体对于有去卵巢大鼠骨折愈合影响的实验研究

发布时间：2018-05-19 04:43

本文选题：去势大鼠 + 骨质疏松性骨折　；参考：《中国骨质疏松杂志》2017年01期

【摘要】：目的探索骨硬化蛋白单克隆抗体(Scl-Ab)对去势大鼠股骨干骨折愈合影响的实验研究。方法 30只健康雌性SD大鼠随机行假手术(Sham,n=5)和切除双侧卵巢(OVX,n=25)手术12w后每组各取5只大鼠处死取股骨行双能X线仪检测确保骨质疏松的建立,随后OVX组大鼠建立左侧股骨干中段骨折模型随机的分成2组:OVX组及骨硬化蛋白单克隆抗体治疗(Scl-Ab)组。术后第1天Scl-Ab组开始给予药物治疗:Scl-Ab皮下注射(剂量25 mg/kg,每周2次)直至8 w,8 w时所有大鼠处死取股骨行Micro-CT、硬组织切片检测及HE组织切片检测。结果 Micro-CT结果显示:Scl-Ab组和OVX组相比,骨折端有较多的新生骨形成,且有较高BV/TV、Tb.Th、Tb.N和较低的Tb.Sp值,比较有明显的统计学意义。HE结果表明Scl-Ab组骨折端有更多的骨痂形成,且骨痂更加成熟。硬组织切片观察结果表明:Scl-Ab组骨折端骨痂矿化速度更快,和OVX组表明有统计学意义。结论 Scl-Ab通过增加骨折端骨量及促进骨组织矿化来治疗去卵巢大鼠股骨中段骨折的愈合。
[Abstract]:Objective to investigate the effect of bone sclerosing protein monoclonal antibody Scl-Abon on femoral shaft fracture healing in ovariectomized rats. Methods Thirty healthy female Sprague-Dawley rats were randomly divided into two groups: sham-operated rats (5) and bilateral ovariectomies (OVX) 25). After 12 weeks of operation, 5 rats in each group were killed and femurs were sacrificed to ensure the establishment of osteoporosis. Then the left femoral shaft fracture model was established in OVX group. The rats were randomly divided into 2 groups: 1% OVX group and 2 groups treated with bone sclerosing protein monoclonal antibody (OVX) group. On the first day after operation, Scl-Ab group was given subcutaneous injection of 25 mg / kg Scl-Ab (25 mg / kg, twice a week) until 8 weeks after 8 weeks of operation, all rats were killed to perform Micro-CTT, hard tissue sections and HE tissue sections. Results compared with OVX group, the Micro-CT results showed that there were more new bone formation, higher BV / TVTV Tb. Thal Tb.N and lower Tb.Sp value in Scl-Ab group than in OVX group. The results showed that there were more callus formation at the fracture end in Scl-Ab group. And the callus is more mature. The results of hard tissue sections showed that the callus mineralization rate of the fracture tip in the Scl-Ab group was faster than that in the OVX group. Conclusion Scl-Ab can increase the bone mass and promote the mineralization of bone tissue to treat the healing of middle femur fracture in ovariectomized rats.
【作者单位】：湖北省荆门市第一人民医院创伤手足外科;
【分类号】：R687.3;R580

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