高脂膳食对肥胖易感和肥胖抵抗大鼠学习记忆能力的影响及机制

发布时间：2018-05-19 13:05

本文选题：高脂膳食 + 肥胖　；参考：《中国生物化学与分子生物学报》2017年05期

【摘要】：经过长期的高脂膳食后并非所有个体都会发生肥胖,还有些个体会产生肥胖抵抗现象。高脂膳食影响海马依赖的学习记忆等认知功能已被广泛证实,但目前关于高脂膳食对肥胖抵抗个体学习记忆能力影响的研究仍较少见。本文旨在对比研究高脂膳食对肥胖易感(obesity-prone,OP)和肥胖抵抗(obesity-resistant,OR)大鼠空间学习记忆能力的影响,并探讨其潜在的可能机制。Morris水迷宫结果显示,肥胖易感大鼠的学习能力显著低于对照大鼠和肥胖抵抗大鼠,但3组大鼠的记忆功能无显著性差异。Western印迹结果显示,与对照组相比,肥胖易感和肥胖抵抗大鼠海马内脑源性神经营养因子(BDNF)、血管内皮细胞生长因子(VEGF)和突触素(SYN)的含量均显著降低,丙二醛(MDA)和白介素1β(IL-1β)的含量均显著升高;且肥胖易感大鼠海马内上述蛋白质含量的变化更明显。免疫荧光染色和激光共聚焦显微镜扫描结果均显示,肥胖易感大鼠的海马神经发生水平显著低于肥胖抵抗大鼠和对照大鼠,但肥胖抵抗大鼠的海马神经发生水平与对照大鼠相比未见显著性变化。这些结果提示,高脂膳食可能是通过降低海马内突触可塑相关蛋白质的表达和神经发生,以及加剧炎症反应来损害肥胖易感大鼠的空间学习能力,而对肥胖抵抗大鼠的学习记忆能力影响不显著。
[Abstract]:After a long-term high-fat diet, not all individuals will develop obesity, and some individuals will develop obesity resistance. The effects of high-fat diet on the learning and memory function of hippocampal dependence have been widely confirmed, but the study on the effects of high-fat diet on the learning and memory ability of obese resistance individuals is still rare. The purpose of this study was to compare the effects of high fat diet on spatial learning and memory ability in obese susceptible rats (OPO) and obesity-resistant rats (ORs), and to explore its possible mechanism. Morris water maze results showed that: 1. The learning ability of obese susceptible rats was significantly lower than that of control rats and obesity resistant rats. However, there was no significant difference in memory function among the three groups. Western blot showed that compared with the control group, the learning ability of the three groups was significantly lower than that of the control group. The contents of brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF) and synaptophysin (SYN) in hippocampus of obese susceptible and obese resistant rats were significantly decreased, while the contents of malondialdehyde (MDA) and IL-1 尾 (IL-1 尾) were significantly increased. The changes of above-mentioned protein contents in hippocampus of obese susceptible rats were more obvious. The results of immunofluorescence staining and laser confocal microscopy showed that the hippocampal neurogenesis of obese susceptible rats was significantly lower than that of obese resistant rats and control rats. However, there was no significant change in hippocampal neurogenesis in obese resistant rats compared with control rats. These results suggest that high-fat diets may impair the spatial learning ability of obese susceptible rats by reducing the expression of synaptic plasticized proteins and neurogenesis in the hippocampus, as well as exacerbating inflammatory responses. However, there was no significant effect on the ability of learning and memory in obese resistant rats.
【作者单位】：上海体育学院运动科学学院;上海健康医学院康复学院;广西民族师范学院;潍坊医学院临床医学院;
【基金】：国家自然科学基金资助(No.81572241) 上海体育学院“运动健身科技”省部共建教育部重点实验室资助项目~~
【分类号】：R589.2

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