BDNF和β干扰素对EAE大鼠胶质细胞生长和免疫因子的影响

发布时间：2018-05-20 03:14

本文选题：BDNF + β干扰素　；参考：《昆明医科大学》2017年硕士论文

【摘要】：[目的]研究脑源性神经生长因子(Brain derived neurotrophic factor,BDNF)对正常SD大鼠(Sprague-Dawley rat)和实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)模型中星形胶质细胞和少突胶质细胞生长的影响,以及BDNF和β干扰素(Beta interferon,βinterferon)对EAE SD大鼠免疫因子的影响,为多发性硬化症(multiple sclerosis,MS)的治疗开辟新思路并提供部分理论依据。[方法]通过不完全弗氏佐剂和结核分枝杆菌诱导EAE动物模型,培养健康对照组和EAE实验组新生大鼠的星形胶质细胞和少突胶质细胞,传代后向其培养液中按浓度滴加 BDNF(0 ng/ml、0.1ng/ml、1ng/ml、10ng/ml)和 β 干扰素(0 ug/ml、0.01ug/ml、0.1ug/ml、1ug/ml),通过免疫荧光观察星形胶质细胞和少突胶质细胞数量的变化(均数±标准差),通过酶联免疫吸附法(ELISA)检测OD450析光度值,通过统计分析,评估抗炎因子白细胞介素-10(interleukin-10,IL-10)、趋化因子(The chemokine C-X-C motif ligand 1,CXCL1)以及促炎因子白细胞介素-1(interleukin-1,IL-1)、肿瘤坏死因子-α(tumornecrosis factor-a,TNF-P)的水平。[结果]实验组和对照组的培养基中加入一定浓度BDNF(0 ng/ml、0.1ng/ml、1ng/ml、10ng/ml)后星形胶质细胞和少突胶质细胞数量是增加的,并且随着浓度的逐渐增加,星形胶质细胞和少突胶质细胞数量是逐渐增加的(均数±标准差)。本实验还发现,在实验组中,一定浓度范围内(0 ng/ml、0.1ng/ml、1ng/ml、10ng/ml)的 BDNF 和 β 干扰素(0ug/ml、0.01ug/ml、0.1ug/ml、1ug/ml),能够增加抗炎因子IL-10和CXCL1的水平(P0.001),同时能够降低促炎因子IL-1和 TNF-P 的水平(P0.001)。[结论]该研究表明了 BDNF能够促进星形胶质细胞和少突胶质细胞的增生,BDNF和β干扰素能够调节炎性反应,有一定的抗炎作用,对MS的治疗可能有一定的价值。
[Abstract]:[objective] to study the effects of brain derived neurotrophic factor (BDNF) on the growth of astrocytes and oligodendrocytes in Sprague-Dawley (SD) rats and experimental autoimmune encephalomyelitis (EAE) models. The effects of BDNF and beta interferon on immune factors in EAE SD rats provide a new idea for the treatment of multiple sclerosis and provide some theoretical basis for the treatment of multiple sclerosis. [methods] EAE animal model was induced by incomplete Freund's adjuvant and Mycobacterium tuberculosis, and astrocytes and oligodendrocytes were cultured in healthy control group and EAE experimental group. After passage, BDNF(0 ng / ml (0.1 ng / ml) and 10 ng / ml 尾 interferon were added to the culture medium according to the concentration. The changes of astrocytes and oligodendrocytes (mean 卤standard deviation) were observed by immunofluorescence, and the luminosity of OD450 was detected by enzyme-linked immunosorbent assay (Elisa), and the changes of astrocytes and oligodendrocytes were observed by immunofluorescence. The levels of interleukin-10 (IL-10), chemokine the chemokine C-X-C motif ligand 1 (CXCL1), interleukin-1 (IL-1) and tumor necrosis factor-1 (TNF- 伪) were evaluated by statistical analysis. [results] the number of astrocytes and oligodendrocytes increased after adding a certain concentration of BDNF(0 ng / ml 0.1 ng / ml to the culture medium of the experimental group and control group, and the number of astrocytes and oligodendrocytes increased with the increase of concentration. The number of astrocytes and oligodendrocytes increased gradually (mean 卤standard deviation). It was also found that in the experimental group, the levels of BDNF and interferon 尾 in the concentration range of 0 ng / ml (0.1 ng / ml) and 10 ng / ml / ml of interferon 尾 were increased by 0.1 ugr / ml / ml, and the levels of anti-inflammatory factor IL-10 and CXCL1 were also increased (P 0.001), and the levels of IL-1 and TNF-P were decreased (P 0.001). [conclusion] this study suggests that BDNF can promote the proliferation of astrocytes and oligodendrocytes. BDNF and interferon 尾 can regulate the inflammatory response, and have some anti-inflammatory effects, which may be valuable for the treatment of MS.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R744.51

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