不同浓度异丙酚对哮喘大鼠氧化应激反应及气道炎症的影响

发布时间：2018-05-22 13:29

本文选题：异丙酚 + 哮喘　；参考：《中国老年学杂志》2017年09期

【摘要】：目的探讨不同浓度异丙酚对哮喘大鼠氧化应激反应及气道炎症的影响。方法清洁级SD大鼠32只随机分为正常组、模型组、低剂量组、高剂量组,每组大鼠8只。正常组给予等量生理盐水灌胃;模型组给予等量生理盐水灌胃,且于22 d雾化吸入后腹腔注射50 mg/kg戊巴比妥钠麻醉;低剂量组、高剂量组分别给予25 mg·kg~(-1)·h~(-1)和50 mg·kg~(-1)·h~(-1),静脉滴注。各组大鼠均在治疗后7 d取器官组织行HE染色观察。比较各组大鼠血清白介素(IL)-5、分离嗜酸性粒细胞(EOS)、免疫球蛋白(Ig)E、IL-4水平及肺组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)水平变化。结果与正常组比较,模型组、低剂量组、高剂量组IL-5、EOS、Ig E和IL-4水平明显增加,差异有统计学意义(P0.05);低剂量组和高剂量组IL-5、EOS、Ig E和IL-4水平明显低于模型组(P0.05);高剂量组IL-5、EOS、Ig E和IL-4水平明显低于低剂量组(P0.05);与正常组比较,模型组、低剂量组、高剂量组MDA水平明显增加,而SOD水平明显降低(P0.05);低剂量组和高剂量组MDA水平明显低于模型组,而SOD水平明显高于模型组(P0.05);高剂量组MDA水平明显低于低剂量组,而SOD水平明显高于低剂量组(P0.05)。结论不同浓度异丙酚可抑制哮喘模型大鼠增高的氧化应激反应,以及缓解大鼠气道炎症,其中以50 mg·kg~(-1)·h~(-1)浓度的异丙酚效果更为明显。
[Abstract]:Objective to investigate the effects of propofol at different concentrations on oxidative stress and airway inflammation in asthmatic rats. Methods Thirty-two clean SD rats were randomly divided into normal group, model group, low dose group and high dose group, with 8 rats in each group. The normal group was given the same amount of normal saline intragastrically, the model group was given the same amount of normal saline, and 50 mg/kg pentobarbital sodium was injected intraperitoneally after 22 days of atomization inhalation. All the rats in each group were observed by HE staining on the 7th day after treatment. Serum IL-5, eosinophil, immunoglobulin IgE, IL-4 and the levels of superoxide dismutase (SOD), malondialdehyde (MDA) MDAA, nitric oxide (no) in lung homogenate were compared in each group. Results compared with the normal group, the levels of IL-5 EOS E and IL-4 increased significantly in the model group, low dose group and high dose group. The levels of Ig E and IL-4 in low dose group and high dose group were significantly lower than those in model group, and the levels of IL-5 EOSG E and IL-4 in high dose group were significantly lower than those in low dose group (P 0.05), and in model group, low dose group, compared with normal group, the level of Ig E and IL-4 in high dose group was significantly lower than that in low dose group. The level of MDA in the high dose group was significantly increased, while the level of SOD was significantly decreased, the MDA level in the low dose group and the high dose group was significantly lower than that in the model group, while the SOD level in the high dose group was significantly higher than that in the model group, and the MDA level in the high dose group was significantly lower than that in the low dose group. The level of SOD was significantly higher than that of low dose group (P 0.05). Conclusion different concentrations of propofol can inhibit the increased oxidative stress response and relieve airway inflammation in asthmatic rats. The effects of propofol at the concentration of 50 mg 路kg ~ (-1) 路h ~ (-1) 路h ~ (-1) are more obvious.
【作者单位】：漯河医学高等专科学校外科教研室;漯河医学高等专科学校;漯河医学高等专科学校内科教研室;漯河医学高等专科学校第一附属医院麻醉科;郑州大学第二附属医院呼吸内科;
【分类号】：R562.25

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