抗SSA抗体阳性干燥综合征患者血清miR-34、miR-500表达变化及其诊断价值

发布时间：2018-05-24 16:42

本文选题：干燥综合征 + 抗SSA抗体　；参考：《山东医药》2017年25期

【摘要】：目的观察抗SSA抗体阳性干燥综合征(p SS)患者血清miR-34、miR-500表达变化,并探讨二者在抗SSA抗体阳性p SS诊断中的临床价值。方法收集p SS患者75例(观察组),非p SS患者84例(对照组),miRNA芯片分析两组血清miRNA表达谱改变;并通过实时荧光定量RT-PCR进行验证。随后应用统计学方法分析异常表达miRNA在抗SSA抗体阳性p SS诊断中的价值。结果 miRNA芯片分析发现6个上调和4个下调的miRNA,其中miR-34和miR-500表达在观察组血清中明显升高;实时荧光定量PCR的结果证实miR-34和miR-500在观察组明显上调,与对照组比较,P0.001。受试者工作曲线分析结果显示,观察组miR-34对p SS诊断的AUC为0.783(95%CI:0.710~0.844,P0.001);miR-500的AUC为0.774(95%CI:0.701~0.837,P0.001)。而miR-34联合miR-500在p SS诊断中AUC为0.888(95%CI:0.828~0.932,P0.001);两者联合诊断p SS的AUC与单一检测比较,P均0.05。结论 miR-34和miR-500在抗SSA抗体阳性p SS患者血清中表达升高,检测血清miR-34和miR-500有助于抗SSA抗体阳性p SS诊断。
[Abstract]:Objective to observe the changes of serum miR-34 miR-500 expression in patients with anti SSA antibody positive Sjogren's syndrome (SSS) and to explore their clinical value in the diagnosis of anti SSA antibody positive PSS. Methods 75 patients with PSS (observation group, 84 patients without PSS) were collected. The changes of serum miRNA expression profiles in two groups were analyzed by miRNA microarray and verified by real-time fluorescence quantitative RT-PCR. Then the value of abnormal expression of miRNA in the diagnosis of anti SSA antibody positive PSS was analyzed by statistical method. Results six upregulated miRNAs and 4 down-regulated miRNAs were found by miRNA microarray analysis, in which the expression of miR-34 and miR-500 was significantly increased in the serum of the observation group, and the results of real-time fluorescence quantitative PCR confirmed that miR-34 and miR-500 were up-regulated in the observation group, compared with the control group (P 0.001). The results of the operating curve analysis showed that the AUC of miR-34 for PSS in the observation group was 0.783 / 95 and the AUC of the observation group was 0.774 / 95CI: 0.701and 0.837 / P0.001respectively. In the diagnosis of PSS, the AUC of miR-34 combined with miR-500 was 0. 888 + 95% CI: 0. 828 and 0. 932% P0.001, and the AUC of the combined diagnosis of PSS was both 0. 05% and 0. 05% compared with that of single detection (P < 0. 05). Conclusion the expression of miR-34 and miR-500 in serum of patients with anti SSA antibody positive PSS is increased. Detection of serum miR-34 and miR-500 is helpful for the diagnosis of anti SSA antibody positive PSS.
【作者单位】：江南大学附属医院·无锡市第四人民医院;无锡市人民医院;杭州师范大学附属医院;
【基金】：亚太风湿病学会联盟基金资助项目(2011) 江苏省自然科学基金青年项目(BK20140171)
【分类号】：R593.2

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