一次性尿液采集器用于尿液HIV-1抗体检测的实验室评估

发布时间：2018-05-25 07:11

  本文选题：一次性尿液采集器 + 尿液　； 参考：《中国艾滋病性病》2017年01期

【摘要】：目的评估一次性尿液采集器保存的尿液样本,用于艾滋病病毒(HIV)抗体检测的可行性。方法利用尿液酶联免疫吸附试验,比较了普通样品管和采集器对尿液HIV-1抗体检出的影响;进一步检测了不同温度和储存时间下,采集器对尿液HIV-1抗体保存的均一性、稳定性以及邮寄前后的影响。结果与普通样品管比较,采集器保存的尿液,对HIV-1抗体检测无明显影响(P=0.24);在不同温度下,采集器可稳定保存尿液的时间:采集器尿液在-20℃和4℃保存1年,在常温保存1个月,在37℃保存9天。均一性:批间和批内变异系数(CV)均15%(P0.05);邮寄前后尿液HIV-1抗体无显著变化,Kappa值为0.957。结论一次性尿液采集器可用于检测HIV-1抗体尿液样本的采集、贮存与运输。
[Abstract]:Objective to evaluate the feasibility of using disposable urine collector to detect HIV antibody. Methods the effects of ordinary sample tubes and collectors on the detection of HIV-1 antibody in urine were compared by Elisa, and the homogeneity of HIV-1 antibody in urine was further examined at different temperature and storage time. Stability and influence before and after mailing. Results compared with the normal sample tube, the urine stored by the collector had no obvious effect on the detection of HIV-1 antibody, and the time of the urine retention was stable at different temperatures: the urine was stored at -20 鈩，

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