hsp70-2基因多态性与钒暴露对工人神经行为功能的影响

发布时间：2018-05-25 21:22

本文选题：钒及其化合物 + 神经行为功能　；参考：《现代预防医学》2016年05期

【摘要】：目的探讨钒暴露与热休克蛋白(Heat Shock Protein,HSP)70-2基因多态性对作业工人神经行为功能的影响。方法采用整群抽样抽取某钒制品厂和某化工厂符合条件的各128例工人作为接钒组和对照组,用神经行为核心测验组合(NCTB)测试研究对象的神经行为功能;通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法来检测作业工人hsp70-2(rs1061581)位点的单核苷酸多态性。结果 (1)在hsp70-2位点上,接钒组和对照组在神经行为功能上的差异主要表现为:在A/G基因型组中,接钒组在错误次数、视觉保留、数字跨度(倒序)、提转捷度(非利手)、目标追踪(正确点数、总打点数)6项上得分更低且差异有统计学意义(P0.05);而在A/A基因型组中,接钒组仅在数字跨度(倒序)上得分更低且差异有统计学意义(P0.05);在G/G基因型组中,接钒组仅在错误次数、数字译码两项上得分更低且有统计学意义(P0.05)。采用主成分分析法构建了神经行为综合指数(Neurobehavioral Index,NBI),在A/G基因型组中,接钒组的NBI得分更低且差异有统计学意义(P0.05)。(2)接钒组在包括提转捷度(非利手),目标追踪(正确点数,总打点数),数字跨度(顺序)以及NBI得分均以带有A/G基因型的一组得分最低,但各基因型间差异均无统计学意义(P0.05),对照组未发现此规律。(3)析因分析未发现钒暴露与hsp70-2多态性存在能影响调查对象神经行为功能的交互作用。结论在神经行为表现上,含有A/G基因型的个体在神经行为表现上更容易受到钒暴露的影响,尚未观察到hsp70-2与钒暴露间存在交互作用。
[Abstract]:Objective to investigate the effects of vanadium exposure and heat shock protein (HSP70-2) gene polymorphism on neurobehavioral function in workers exposed to vanadium. Methods one hundred and twenty-eight workers from a vanadium factory and a chemical factory were selected by cluster sampling as the vanadium group and the control group. The neurobehavioral function of the subjects was tested by NCTB. The single nucleotide polymorphism at the hsp70-2rs1061581) site was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results (1) at the hsp70-2 site, the difference of neurobehavioral function between vanadium group and control group was as follows: in the Ar / G genotype group, in the wrong number of times, the visual retention was observed in the vanadium group. The number span (reverse order), the speed of transfer (not handy), the target tracking (correct point, total score 6) were lower and the difference was statistically significant (P < 0.05), while in the A / A genotype group, the score was lower than that in the A / A genotype group, but in the A / A genotype group, the score was lower and the difference was statistically significant (P < 0.05). In the G / G genotype group, the vanadium group only scored lower on the number span (in reverse order) and the difference was statistically significant (P 0.05), while in the G / G genotype group, the vanadium group only scored lower on the wrong times, and the two items of digital decoding were lower and had statistical significance (P 0.05). The neurobehavioral index (NBI) was constructed by principal component analysis (PCA). In the A / G genotype group, the NBI score of the vanadium group was lower and the difference was statistically significant (P 0.05). The number span (order) and the NBI score were the lowest in the group with the A / G genotype. However, there was no significant difference among all genotypes (P 0.05), and no such rule was found in the control group (P < 0.05). No interaction between vanadium exposure and hsp70-2 polymorphism was found in the control group, which could affect the neurobehavioral function of the subjects investigated. Conclusion in neurobehavioral behavior, individuals with A / G genotype are more susceptible to vanadium exposure, and no interaction between hsp70-2 and vanadium exposure has been observed.
【作者单位】：四川大学华西公共卫生学院(华西第四医院);深圳市龙岗区疾病预防控制中心职业病防治科;攀枝花市第二人民医院;四川大学华西基础医学与法医学院生物化学与分子生物学教研室;
【基金】：攀枝花市主要职业病防治相关技术服务及分子遗传标志物检测分析(编号:11H1219)
【分类号】：R135.1

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